Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박혜경 | * |
dc.contributor.author | 정영해 | * |
dc.date.accessioned | 2016-08-28T11:08:37Z | - |
dc.date.available | 2016-08-28T11:08:37Z | - |
dc.date.issued | 2001 | * |
dc.identifier.issn | 1355-0284 | * |
dc.identifier.other | OAK-12561 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/228677 | - |
dc.description.abstract | To elucidate the pathogenic mechanisms involved in neurodegeneration in AIDS patients with cognitive deficits, we have examined the toxic effect of the lentivirus lytic peptide 1 (LLP-1) corresponding to the carboxyl terminus of HIV-1 transmembrane glycoprotein gp41 on human neuronal and glial cell lines. LLP-1 induced a significant lactate dehydrogenase (LDH, a marker of cell death) release from these cells in a concentration- and time-dependent manner, while the noncytolytic LLP-1 analog 2 had little effect. Application of LLP-1 to SH-SY5Y, a well-characterized human neuronal cell line, caused the decline of intracellular glutathione (GSH) content that appeared to occur before a significant LDH release. Furthermore, LLP-1 elicited a significant loss of mitochondrial function as measured by mitochondrial transmembrane potential (MTP). Among the reducing agents and antioxidants tested, GSH and a GSH prodrug N-acetylcysteine (NAC) provided protection against LLP-1-induced neuronal cell death, evidently by restoring the intracellular GSH levels and blocking the disruption of mitochondrial integrity. Thus, gp41-derived LLP-1 may be a potential neurotoxic agent capable of causing the intracellular GSH depletion and disturbing the mitochondrial function, possibly contributing to the neurodegenerative cascade as seen in HIV-1-associated dementia. Our data indicate that restoring both GSH concentration and mitochondrial function may hold promise as possible therapeutic strategies for slowing disease progression of dementia in AIDS patients. | * |
dc.language | English | * |
dc.title | Protective effect of glutathione in HIV-1 lytic peptide 1-induced cell death in human neuronal cells | * |
dc.type | Article | * |
dc.relation.issue | 5 | * |
dc.relation.volume | 7 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 454 | * |
dc.relation.lastpage | 465 | * |
dc.relation.journaltitle | Journal of NeuroVirology | * |
dc.identifier.doi | 10.1080/135502801753170318 | * |
dc.identifier.wosid | WOS:000171755800008 | * |
dc.identifier.scopusid | 2-s2.0-0034754762 | * |
dc.author.google | Sung J.H. | * |
dc.author.google | Shin S.A. | * |
dc.author.google | Park H.K. | * |
dc.author.google | Montelaro R.C. | * |
dc.author.google | Chong Y.H. | * |
dc.contributor.scopusid | 박혜경(36014150800) | * |
dc.contributor.scopusid | 정영해(7201371824) | * |
dc.date.modifydate | 20240423081003 | * |