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(-)-Epicatechin gallate (ECG) stimulates osteoblast differentiation via runt-related transcription factor 2 (RUNX2) and transcriptional coactivator with PDZ-binding motif (TAZ)-mediated transcriptional activation

Title
(-)-Epicatechin gallate (ECG) stimulates osteoblast differentiation via runt-related transcription factor 2 (RUNX2) and transcriptional coactivator with PDZ-binding motif (TAZ)-mediated transcriptional activation
Authors
Byun M.R.Sung M.K.Kim A.R.Lee C.H.Jang E.J.Jeong M.G.Noh M.Hwang E.S.Hong J.-H.
Ewha Authors
황은숙
SCOPUS Author ID
황은숙scopus
Issue Date
2014
Journal Title
Journal of Biological Chemistry
ISSN
0021-9258JCR Link
Citation
Journal of Biological Chemistry vol. 289, no. 14, pp. 9926 - 9935
Publisher
American Society for Biochemistry and Molecular Biology Inc.
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Osteoporosis is a degenerative bone disease characterized by low bone mass and is caused by an imbalance between osteoblastic bone formation and osteoclastic bone resorption. It is known that the bioactive compounds present in green tea increase osteogenic activity and decrease the risk of fracture by improving bone mineral density. However, the detailed mechanism underlying these beneficial effects has yet to be elucidated. In this study, we investigated the osteogenic effect of (-)-epicatechin gallate (ECG), a major bioactive compound found in green tea. We found that ECG effectively stimulates osteoblast differentiation, indicated by the increased expression of osteoblastic marker genes. Up-regulation of osteoblast marker genes is mediated by increased expression and interaction of the transcriptional coactivator with PDZ-binding motif (TAZ) and Runt-related transcription factor 2 (RUNX2). ECG facilitates nuclear localization ofTAZthrough PP1A. PP1A is essential for osteoblast differentiation because inhibition of PP1A activity was shown to suppress ECG-mediated osteogenic differentiation. Taken together, the results showed that ECG stimulates osteoblast differentiation through the activation of TAZ and RUNX2, revealing a novel mechanism for green tea-stimulated osteoblast differentiation. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
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DOI
10.1074/jbc.M113.522870
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약학대학 > 약학과 > Journal papers
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