Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 강동민 | - |
dc.date.accessioned | 2016-08-28T10:08:15Z | - |
dc.date.available | 2016-08-28T10:08:15Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0143-3334 | - |
dc.identifier.other | OAK-10294 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223898 | - |
dc.description.abstract | Hepatitis B virus (HBV) X prote in (HBx), encoded by the HBV genome, is involved in the development of HBV-mediated liver cancer, whose frequency is highly correlated with chromosomal instability (CIN). We reported previously that HBx induces mitotic checkpoint dysfunction by targeting the human serine/threonine kinase BubR1 (hBubR1). However, the underlying mechanism remained unresolved. Here, we show that HBx protein-associated protein a (HBxAPa)/Rsf-1 associates with hBubR1 and HBx in the chromatin fraction during mitosis. Depletion of HBxAPa/Rsf-1 abolished the interaction between HBx and hBubR1, indicating that HBxAPa/Rsf-1 mediates these interactions. Knockdown of HBxAPa/Rsf-1 with small interfering RNA did not affect the recruitment of hBubR1 to kinetochores; however, it did significantly impair HBx targeting to kinetochores. A deletion mutant analysis revealed that two Kunitz domains of HBx, the Cdc20-binding domain of hBubR1 and full-length of HBxAPa/Rsf-1 were essential for these interactions. Thus, binding of HBx to hBubR1, stabilized by HBxAPa/Rsf-1, significantly attenuated hBubR1 binding to Cdc20 and consequently increased the rate of mitotic aberrations. Collectively, our data show that the HBx impairs hBubR1 function and induces CIN through HBxAPa/Rsf-1, providing a novel mechanism for induction of genomic instability by a viral pathogen in hepatocarcinogenesis. © The Author 2013. Published by Oxford University Press. All rights reserved. | - |
dc.language | English | - |
dc.title | HBxAPα/Rsf-1-mediated HBx-hBubR1 interactions regulate the mitotic spindle checkpoint and chromosome instability | - |
dc.type | Article | - |
dc.relation.issue | 7 | - |
dc.relation.volume | 34 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1680 | - |
dc.relation.lastpage | 1688 | - |
dc.relation.journaltitle | Carcinogenesis | - |
dc.identifier.doi | 10.1093/carcin/bgt105 | - |
dc.identifier.wosid | WOS:000321753000032 | - |
dc.identifier.scopusid | 2-s2.0-84880263801 | - |
dc.author.google | Chae S. | - |
dc.author.google | Ji J.-H. | - |
dc.author.google | Kwon S.-H. | - |
dc.author.google | Lee H.-S. | - |
dc.author.google | Lim J.M. | - |
dc.author.google | Kang D. | - |
dc.author.google | Lee C.-W. | - |
dc.author.google | Cho H. | - |
dc.contributor.scopusid | 강동민(13103841000) | - |
dc.date.modifydate | 20230210131016 | - |