View : 271 Download: 0
Expression of the ammonia transporter family member, Rh B Glycoprotein, in the human kidney
- Expression of the ammonia transporter family member, Rh B Glycoprotein, in the human kidney
- Han K.-H.; Lee H.-W.; Handlogten M.E.; Whitehill F.; Osis G.; Croker B.P.; Clapp W.L.; Verlander J.W.; Weiner I.D.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- American Journal of Physiology - Renal Physiology
- American Journal of Physiology - Renal Physiology vol. 304, no. 7, pp. F972 - F981
- Document Type
Show the fulltext
- The ammonia transporter family member, Rh B Glycoprotein (RhBG/Rhbg), is essential for ammonia transport by the rodent kidney, but in the human kidney mRNA but not protein expression has been reported. Because ammonia transport is fundamental for acid-base homeostasis, the current study addressed RhBG expression in the human kidney. Two distinct RhBG mRNA sequences have been reported, with different numbers of consecutive cytosines at nt1265 and thus encoding different car-boxy-tails. Sequencing the region of difference in both human kidney and liver mRNA showed eight sequential cytosines, not seven as in some reports. Knowing the correct mRNA sequence for RhBG, we then assessed RhBG protein expression using antibodies against the correct amino acid sequence. Immunoblot analysis demonstrated RhBG protein expression in human kidney and immunohistochemis-try identified basolateral RhBG in connecting segment (CNT) and the cortical and outer medullary collecting ducts. Colocalization of RhBG with multiple cell-specific markers demonstrated that that CNT cells and collecting duct type A intercalated cells express high levels of RhBG, and type B intercalated cells and principal cells do not express detectable RhBG. Thus, these studies identify the correct mRNA and thus protein sequence for human RhBG and show that the human kidney expresses basolateral RhBG protein in CNT, type A intercalated cells, and non-A, non-B cells. We conclude that RhBG can mediate an important role in human renal ammonia transport. © 2013 the American Physiological Society.
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.