Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이공주 | - |
dc.contributor.author | 신동해 | - |
dc.date.accessioned | 2016-08-28T10:08:42Z | - |
dc.date.available | 2016-08-28T10:08:42Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0907-4449 | - |
dc.identifier.other | OAK-9920 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223577 | - |
dc.description.abstract | Nm23-H1/NDPK-A, a tumour metastasis suppressor, is a multifunctional housekeeping enzyme with nucleoside diphosphate kinase activity. Hexameric Nm23-H1 is required for suppression of tumour metastasis and it is dissociated into dimers under oxidative conditions. Here, the crystal structure of oxidized Nm23-H1 is presented. It reveals the formation of an intramolecular disulfide bond between Cys4 and Cys145 that triggers a large conformational change that destabilizes the hexameric state. The dependence of the dissociation dynamics on the H2O2 concentration was determined using hydrogen/deuterium-exchange experiments. The quaternary conformational change provides a suitable environment for the oxidation of Cys109 to sulfonic acid, as demonstrated by peptide sequencing using nanoUPLC-ESI-q-TOF tandem MS. From these and other data, it is proposed that the molecular and cellular functions of Nm23-H1 are regulated by a series of oxidative modifications coupled to its oligomeric states and that the modified cysteines are resolvable by NADPH-dependent reduction systems. These findings broaden the understanding of the complicated enzyme-regulatory mechanisms that operate under oxidative conditions. © 2013 International Union of Crystallography Printed in Singapore - all rights reserved. | - |
dc.language | English | - |
dc.title | Structure of Nm23-H1 under oxidative conditions | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 69 | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 669 | - |
dc.relation.lastpage | 680 | - |
dc.relation.journaltitle | Acta Crystallographica Section D: Biological Crystallography | - |
dc.identifier.doi | 10.1107/S0907444913001194 | - |
dc.identifier.wosid | WOS:000316743300020 | - |
dc.identifier.scopusid | 2-s2.0-84875478027 | - |
dc.author.google | Kim M.-S. | - |
dc.author.google | Jeong J. | - |
dc.author.google | Shin D.-H. | - |
dc.author.google | Lee K.-J. | - |
dc.contributor.scopusid | 이공주(7501497635;57191532162) | - |
dc.contributor.scopusid | 신동해(57217374185) | - |
dc.date.modifydate | 20230208115507 | - |