Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 심현보 | * |
dc.date.accessioned | 2016-08-28T10:08:38Z | - |
dc.date.available | 2016-08-28T10:08:38Z | - |
dc.date.issued | 2013 | * |
dc.identifier.issn | 0253-2964 | * |
dc.identifier.other | OAK-9883 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223542 | - |
dc.description.abstract | Single-chain variable fragments of antibodies (scFv) specific to 2,4-dinitrotoluene (DNT) were isolated from a phage library displaying synthetic human scFv fragments with 6 diversified complementary determining regions (CDRs). A DNT derivative that contained an extended amine group was synthesized and conjugated to the NHS-group that was linked to magnetic beads. Phages specific to the immobilized DNT derivatives were isolated from the library after 4 rounds of sequential binding and elution processes. The displayed scFv fragments from the isolated phages showed consensus CDR sequences. One DNT-specific scFv was expressed in E. coli and purified using Ni-affinity chromatography. The purified DNT-specific scFv binds specifically to the immobilized DNT-derivative with KD value of 6.0 × 10?7 M. The scFv and DNT interaction was not disrupted by the addition of 4-nitrotoluene or benzoic acid. These data demonstrate that the screened scFv from the phage displayed library could be used for selective and sensitive detection of explosives such as TNT. | * |
dc.language | English | * |
dc.title | Development of a single chain antibody using a phage display cloning method for the detection of 2,4-dinitrotoluene | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 34 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 460 | * |
dc.relation.lastpage | 464 | * |
dc.relation.journaltitle | Bulletin of the Korean Chemical Society | * |
dc.identifier.doi | 10.5012/bkcs.2013.34.2.460 | * |
dc.identifier.wosid | WOS:000316430200025 | * |
dc.identifier.scopusid | 2-s2.0-84874537154 | * |
dc.author.google | Na J.-H. | * |
dc.author.google | Joo M.-S. | * |
dc.author.google | Lee W.-K. | * |
dc.author.google | Shim H. | * |
dc.author.google | Lim S.-H. | * |
dc.author.google | Taek Jung S. | * |
dc.author.google | Gyu Yu Y. | * |
dc.contributor.scopusid | 심현보(26635827900) | * |
dc.date.modifydate | 20240123110611 | * |