Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 권영주 | * |
dc.date.accessioned | 2016-08-28T10:08:35Z | - |
dc.date.available | 2016-08-28T10:08:35Z | - |
dc.date.issued | 2013 | * |
dc.identifier.issn | 1082-6068 | * |
dc.identifier.other | OAK-9851 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223516 | - |
dc.description.abstract | For HER2 overexpression, the ESX transcription factor must interact with both the HER2 promoter and Sur2, a subunit of human mediator complex, using its Ets domain and transactivation domain, respectively. HER2 overexpression is a marker of poor prognosis in various types of cancers. Thus, interfering with the ESX-Sur2 interaction has been suggested as a novel strategy for the treatment of HER2 positive cancers. To design small molecule inhibitors against the ESX-Sur2 interaction, it is necessary to identify the structure of the interface of ESX-Sur2 binding. Therefore, in this study, we determined the optimal conditions for the overexpression and purification of a new version of Sur2, Sur2391-582, which was able to bind to ESX. To stabilize (His) 6-Sur2391-582, various different buffered conditions over a wide range of pH and ionic strengths were examined. The molecular mass of (His)6-Sur2391-582 was determined using mass spectroscopy and size exclusion chromatography. The purified (His)6-Sur 2391-582 protein displayed the same biological properties as that of the Sur2 full-length protein. © 2013 Taylor & Francis Group, LLC. | * |
dc.language | English | * |
dc.title | Expression and purification of a soluble ESX-binding core domain of sur2 | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 43 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 364 | * |
dc.relation.lastpage | 375 | * |
dc.relation.journaltitle | Preparative Biochemistry and Biotechnology | * |
dc.identifier.doi | 10.1080/10826068.2012.738273 | * |
dc.identifier.wosid | WOS:000316065600004 | * |
dc.identifier.scopusid | 2-s2.0-84875516718 | * |
dc.author.google | Jeon K.-H. | * |
dc.author.google | Jun K.-Y. | * |
dc.author.google | Kim E.Y. | * |
dc.author.google | Kwon Y. | * |
dc.contributor.scopusid | 권영주(12446435600) | * |
dc.date.modifydate | 20240422124907 | * |