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dc.contributor.author권영주*
dc.date.accessioned2016-08-28T10:08:35Z-
dc.date.available2016-08-28T10:08:35Z-
dc.date.issued2013*
dc.identifier.issn1082-6068*
dc.identifier.otherOAK-9851*
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/223516-
dc.description.abstractFor HER2 overexpression, the ESX transcription factor must interact with both the HER2 promoter and Sur2, a subunit of human mediator complex, using its Ets domain and transactivation domain, respectively. HER2 overexpression is a marker of poor prognosis in various types of cancers. Thus, interfering with the ESX-Sur2 interaction has been suggested as a novel strategy for the treatment of HER2 positive cancers. To design small molecule inhibitors against the ESX-Sur2 interaction, it is necessary to identify the structure of the interface of ESX-Sur2 binding. Therefore, in this study, we determined the optimal conditions for the overexpression and purification of a new version of Sur2, Sur2391-582, which was able to bind to ESX. To stabilize (His) 6-Sur2391-582, various different buffered conditions over a wide range of pH and ionic strengths were examined. The molecular mass of (His)6-Sur2391-582 was determined using mass spectroscopy and size exclusion chromatography. The purified (His)6-Sur 2391-582 protein displayed the same biological properties as that of the Sur2 full-length protein. © 2013 Taylor & Francis Group, LLC.*
dc.languageEnglish*
dc.titleExpression and purification of a soluble ESX-binding core domain of sur2*
dc.typeArticle*
dc.relation.issue4*
dc.relation.volume43*
dc.relation.indexSCI*
dc.relation.indexSCIE*
dc.relation.indexSCOPUS*
dc.relation.startpage364*
dc.relation.lastpage375*
dc.relation.journaltitlePreparative Biochemistry and Biotechnology*
dc.identifier.doi10.1080/10826068.2012.738273*
dc.identifier.wosidWOS:000316065600004*
dc.identifier.scopusid2-s2.0-84875516718*
dc.author.googleJeon K.-H.*
dc.author.googleJun K.-Y.*
dc.author.googleKim E.Y.*
dc.author.googleKwon Y.*
dc.contributor.scopusid권영주(12446435600)*
dc.date.modifydate20240422124907*
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약학대학 > 약학과 > Journal papers
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