Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 하헌주 | * |
dc.date.accessioned | 2016-08-28T10:08:09Z | - |
dc.date.available | 2016-08-28T10:08:09Z | - |
dc.date.issued | 2012 | * |
dc.identifier.issn | 0168-8227 | * |
dc.identifier.other | OAK-9536 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223250 | - |
dc.description.abstract | Aims: The present study examined renoprotective effect of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSC) in diabetes. NRK-52E cells were utilized to determine the paracrine effect of hUCB-MSC. Methods: hUCB was harvested with the mother's consent. MSC obtained from the hUCB were injected through the tail vein. Growth arrested and synchronized NRK-52E cells were stimulated with transforming growth factor-β1 (TGF-β1) in the presence of hUCB-MSC conditioned media. Results: At 4 weeks after the streptozotocin (STZ) injection, diabetic rats showed significantly increased urinary protein excretion, renal and glomerular hypertrophy, fractional mesangial area, renal expression of TGF-β1 and α-smooth muscle actin, and collagen accumulation but decreased renal E-cadherin and bone morphogenic protein-7 expression, confirming diabetic renal injury. hUCB-MSC effectively prevented diabetic renal injury except renal and glomerular hypertrophy without a significant effect on blood glucose. CM-DiI-labeled hUCB-MSC and immunostaining of PKcs, a human nuclei antigen, confirmed a few engraftment of hUCB-MSC in diabetic kidneys. hUCB-MSC conditioned media inhibited TGF-β1-induced extracellular matrix upregulation and epithelial-to-mesenchymal transition in NRK-52E cells in a concentration-dependent manner. Conclusions: These results demonstrate the renoprotective effect of hUCB-MSC in STZ-induced diabetic rats possibly through secretion of humoral factors and suggest hUCB-MSC as a possible treatment modality for diabetic renal injury. © 2012 Elsevier Ireland Ltd. | * |
dc.language | English | * |
dc.title | Human umbilical cord blood-derived mesenchymal stem cells prevent diabetic renal injury through paracrine action | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 98 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 465 | * |
dc.relation.lastpage | 473 | * |
dc.relation.journaltitle | Diabetes Research and Clinical Practice | * |
dc.identifier.doi | 10.1016/j.diabres.2012.09.034 | * |
dc.identifier.wosid | WOS:000312133700020 | * |
dc.identifier.scopusid | 2-s2.0-84870673962 | * |
dc.author.google | Park J.H. | * |
dc.author.google | Hwang I. | * |
dc.author.google | Hwang S.H. | * |
dc.author.google | Han H. | * |
dc.author.google | Ha H. | * |
dc.contributor.scopusid | 하헌주(7202277106) | * |
dc.date.modifydate | 20240123091420 | * |