View : 667 Download: 0

Mesenchymal stem cells restore CCl4-induced liver injury by an antioxidative process

Title
Mesenchymal stem cells restore CCl4-induced liver injury by an antioxidative process
Authors
Cho K.-A.Woo S.-Y.Seoh J.-Y.Han H.-S.Ryu K.-H.
Ewha Authors
서주영유경하우소연조경아
SCOPUS Author ID
서주영scopusscopus; 유경하scopus; 우소연scopus; 조경아scopus
Issue Date
2013
Journal Title
Cell Biology International
ISSN
1065-6995JCR Link
Citation
Cell Biology International vol. 36, no. 12, pp. 1267 - 1274
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
We have investigated BM (bone marrow)-derived MSCs (mesenchymal stem cells) for the treatment of liver injury. It was hypothesized that MSC-mediated resolution of liver injury could occur through an antioxidative process. After being injected with CCl4 (carbon tetrachloride), mice were injected with syngenic BM-derived MSCs or normal saline. Oxidative stress activity of the MSCs was determined by the analysis of ROS (reactive oxygen species) and SOD (superoxide dismutase) activity. In addition, cytoprotective genes of the liver tissue were assessed by real-time PCR and ARE (antioxidant-response element) reporter assay. Up-regulated ROS of CCl4-treated liver cells was attenuated by co-culturing with MSCs. Suppression of SOD by adding an SOD inhibitor decreased the effect of MSCs on injured liver cells. MSCs significantly increased SOD activity and inhibited ROS production in the injured liver. The gene expression levels of Hmox-1 (haem oxygenase-1), BI-1 (Bax inhibitor-1), HGF (hepatocyte growth factor), GST (glutathione transferase) and Nrf2 (nuclear factor-erythoid 2 p45 subunit-related factor 20), attenuated by CCl4, were increased up to basal levels after MSC transplantation. In addition, MSCs induced an ARE, shown by luciferase activity, which represented a cytoprotective response in the injured liver. Evidence of a new cytoprotective effect is shown in which MSCs promote an antioxidant response and supports the potential of using MSC transplantation as an effective treatment modality for liver disease. © The Author(s) Journal compilation. © 2012 International Federation for Cell Biology.
DOI
10.1042/CBI20110634
Appears in Collections:
의과대학 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

BROWSE