Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김주영 | - |
dc.date.accessioned | 2016-08-28T10:08:55Z | - |
dc.date.available | 2016-08-28T10:08:55Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.other | OAK-9320 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223099 | - |
dc.description.abstract | The activity of the serine protease in the German cockroach allergen is important to the development of allergic disease. The protease-activated receptor (PAR)-2, which is expressed in numerous cell types in lung tissue, is known to mediate the cellular events caused by inhaled serine protease. Alveolar macrophages express PAR-2 and produce considerable amounts of tumor necrosis factor (TNF)-α. We determined whether the serine protease in German cockroach extract (GCE) enhances TNF-α production by alveolar macrophages through the PAR-2 pathway and whether the TNF-α production affects GCE-induced pulmonary inflammation. Effects of GCE on alveolar macrophages and TNF-α production were evaluated using in vitro MH-S and RAW264.6 cells and in vivo GCE-induced asthma models of BALB/c mice. GCE contained a large amount of serine protease. In the MH-S and RAW264.7 cells, GCE activated PAR-2 and thereby produced TNF-α. In the GCE-induced asthma model, intranasal administration of GCE increased airway hyperresponsiveness (AHR), inflammatory cell infiltration, productions of serum immunoglobulin E, interleukin (IL)-5, IL-13 and TNF-α production in alveolar macrophages. Blockade of serine proteases prevented the development of GCE induced allergic pathologies. TNF-α blockade also prevented the development of such asthma-like lesions. Depletion of alveolar macrophages reduced AHR and intracellular TNF-α level in pulmonary cell populations in the GCE-induced asthma model. These results suggest that serine protease from GCE affects asthma through an alveolar macrophage and TNF-α dependent manner, reflecting the close relation of innate and adaptive immune response in allergic asthma model. © 2012 Kim et al. | - |
dc.language | English | - |
dc.title | Alveolar Macrophages Play a Key Role in Cockroach-Induced Allergic Inflammation via TNF-α Pathway | - |
dc.type | Article | - |
dc.relation.issue | 10 | - |
dc.relation.volume | 7 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.journaltitle | PLoS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0047971 | - |
dc.identifier.wosid | WOS:000310050200054 | - |
dc.identifier.scopusid | 2-s2.0-84867682725 | - |
dc.author.google | Kim J.Y. | - |
dc.author.google | Sohn J.H. | - |
dc.author.google | Choi J.-M. | - |
dc.author.google | Lee J.-H. | - |
dc.author.google | Hong C.-S. | - |
dc.author.google | Lee J.-S. | - |
dc.author.google | Park J.-W. | - |
dc.contributor.scopusid | 김주영(36152545300;57221537444) | - |
dc.date.modifydate | 20230118111324 | - |