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dc.contributor.author이지희-
dc.contributor.author최윤희-
dc.date.accessioned2016-08-28T12:08:42Z-
dc.date.available2016-08-28T12:08:42Z-
dc.date.issued2011-
dc.identifier.issn1226-3613-
dc.identifier.otherOAK-8297-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/222215-
dc.description.abstractRecent evidence supports a neuroprotective role of Src homology 2-containing protein tyrosine phosphatase 2 (SHP-2) against ischemic brain injury. However, the molecular mechanisms of SHP-2 activation and those governing how SHP-2 exerts its function under oxidative stress conditions are not well understood. Recently we have reported that reactive oxygen species (ROS)-mediated oxidative stress promotes the phosphorylation of endogenous SHP-2 through lipid rafts, and that this phosphorylation strongly occurs in astrocytes, but not in microglia. To investigate the molecules involved in events leading to phosphorylation of SHP-2, raft proteins were analyzed using astrocytes and microglia. Interestingly, caveolin-1 and -2 were detected only in astrocytes but not in microglia, whereas flotillin-1 was expressed in both cell types. To examine whether the H 2O 2-dependent phosphorylation of SHP-2 is mediated by caveolin-1, we used specific small interfering RNA (siRNA) to downregulate caveolin- 1 expression. In the presence of caveolin-1 siRNA, the level of SHP-2 phosphorylation induced by H 2O 2 was significantly decreased, compared with in the presence of control siRNA. Overexpression of caveolin- 1 effectively increased H 2O 2-induced SHP-2 phosphorylation in microglia. Lastly, H 2O 2 induced extracellular signal-regulated kinase (ERK) activation in astrocytes through caveolin-1. Our results suggest that caveolin-1 is involved in astrocyte-specific intracellular responses linked to the SHP-2-mediated signaling cascade following ROS-induced oxidative stress.-
dc.languageEnglish-
dc.titleCaveolin-1 is involved in reactive oxygen species-induced SHP-2 activation in astrocytes-
dc.typeArticle-
dc.relation.issue12-
dc.relation.volume43-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.indexKCI-
dc.relation.startpage660-
dc.relation.lastpage668-
dc.relation.journaltitleExperimental and Molecular Medicine-
dc.identifier.doi10.3858/emm.2011.43.12.075-
dc.identifier.wosidWOS:000298576900002-
dc.identifier.scopusid2-s2.0-84855173802-
dc.author.googleYun J.H.-
dc.author.googlePark S.J.-
dc.author.googleJo A.-
dc.author.googleKang J.L.-
dc.author.googleJou I.-
dc.author.googlePark J.S.-
dc.author.googleChoi Y.-H.-
dc.contributor.scopusid이지희(7404517577)-
dc.contributor.scopusid최윤희(7404776849)-
dc.date.modifydate20190302081000-
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의과대학 > 의학과 > Journal papers
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