Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서주영 | * |
dc.contributor.author | 우소연 | * |
dc.date.accessioned | 2016-08-28T12:08:09Z | - |
dc.date.available | 2016-08-28T12:08:09Z | - |
dc.date.issued | 2011 | * |
dc.identifier.issn | 0022-1767 | * |
dc.identifier.other | OAK-7893 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221892 | - |
dc.description.abstract | Eosinophils are abundant in the lamina propria of the small intestine, but they rarely show degranulation in situ under steady-state conditions. In this study, using two novel mAbs, we found that intestinal eosinophils constitutively expressed a high level of an inhibitory receptor signal regulatory protein α (SIRPα)/CD172a and a low, but significant, level of a tetraspanin CD63, whose upregulation is closely associated with degranulation. Cross-linking SIRPα/CD172a on the surface of wild-type eosinophils significantly inhibited the release of eosinophil peroxidase induced by the calcium ionophore A23187, whereas this cross-linking effect was not observed in eosinophils isolated from mice expressing a mutated SIRPα/CD172a that lacks most of its cytoplasmic domain (SIRPα Cyto -/-). The SIRPα Cyto -/- eosinophils showed reduced viability, increased CD63 expression, and increased eosinophil peroxidase release with or without A23187 stimulation in vitro. In addition, SIRPα Cyto -/- mice showed increased frequencies of Annexin V-binding eosinophils and free MBP +CD63 + extracellular granules, as well as increased tissue remodeling in the small intestine under steady-state conditions. Mice deficient in CD47, which is a ligand for SIRPα/CD172a, recapitulated these phenomena. Moreover, during Th2-biased inflammation, increased eosinophil cell death and degranulation were obvious in a number of tissues, including the small intestine, in the SIRPa Cyto -/- mice compared with wild-type mice. Collectively, our results indicated that SIRPα/CD172a regulates eosinophil homeostasis, probably by interacting with CD47, with substantial effects on eosinophil survival. Thus, SIRPα/CD172a is a potential therapeutic target for eosinophil-associated diseases. Copyright © 2011 by The American Association of Immunologists, Inc. | * |
dc.language | English | * |
dc.title | SIRPα/CD172α regulates eosinophil homeostasis | * |
dc.type | Article | * |
dc.relation.issue | 5 | * |
dc.relation.volume | 187 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2268 | * |
dc.relation.lastpage | 2277 | * |
dc.relation.journaltitle | Journal of Immunology | * |
dc.identifier.doi | 10.4049/jimmunol.1101008 | * |
dc.identifier.wosid | WOS:000294059500031 | * |
dc.identifier.scopusid | 2-s2.0-80052688904 | * |
dc.author.google | Garcia N.V. | * |
dc.author.google | Umemoto E. | * |
dc.author.google | Saito Y. | * |
dc.author.google | Yamasaki M. | * |
dc.author.google | Hata E. | * |
dc.author.google | Matozaki T. | * |
dc.author.google | Murakami M. | * |
dc.author.google | Jung Y.-J. | * |
dc.author.google | Woo S.-Y. | * |
dc.author.google | Seoh J.-Y. | * |
dc.author.google | Jang M.H. | * |
dc.author.google | Aozasa K. | * |
dc.author.google | Miyasaka M. | * |
dc.contributor.scopusid | 서주영(6603709174;57209001625) | * |
dc.contributor.scopusid | 우소연(7402853365) | * |
dc.date.modifydate | 20240118164942 | * |