Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 최정윤 | - |
dc.date.accessioned | 2016-08-28T12:08:40Z | - |
dc.date.available | 2016-08-28T12:08:40Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0006-2960 | - |
dc.identifier.other | OAK-6887 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/221036 | - |
dc.description.abstract | 3-(2′-Deoxy-β-d-erythro-pentofuranosyl)pyrimido-[1,2-a] purin-10(3H)-one (M 1dG) is the major adduct derived from the reaction of DNA with the lipid peroxidation product malondialdehyde and the DNA peroxidation product base propenal. M 1dG is mutagenic in Escherichia coli and mammalian cells, inducing base-pair substitutions (M 1dG → A and M 1dG → T) and frameshift mutations. Y-family polymerases may contribute to the mutations induced by M 1dG in vivo. Previous reports described the bypass of M 1dG by DNA polymerases η and Dpo4. The present experiments were conducted to evaluate bypass of M 1dG by the human Y-family DNA polymerases κ, τ, and Rev1. M 1dG was incorporated into template-primers containing either dC or dT residues 5′ to the adduct, and the template-primers were subjected to in vitro replication by the individual DNA polymerases. Steady-state kinetic analysis of single nucleotide incorporation indicates that dCMP is most frequently inserted by hPol κ opposite the adduct in both sequence contexts, followed by dTMP and dGMP. dCMP and dTMP were most frequently inserted by hPol τ, and only dCMP was inserted by Rev1. hPol κ extended template-primers in the order M 1dG:dC > M 1dG:dG > M 1dG:dT ∼ M 1dG:dA, but neither hPol τ nor Rev1 extended M 1dG-containing template-primers. Liquid chromatography-mass spectrometry analysis of the products of hPol κ-catalyzed extension verified this preference in the 3′-GXC-5′ template sequence but revealed the generation of a series of complex products in which dAMP is incorporated opposite M 1dG in the 3′-GXT-5′ template sequence. The results indicate that DNA hPol κ or the combined action of hPol τ or Rev1 and hPol κ bypass M 1dG residues in DNA and generate products that are consistent with some of the mutations induced by M 1dG in mammalian cells. © 2010 American Chemical Society. | - |
dc.language | English | - |
dc.title | In vitro bypass of the major malondialdehyde-and base propenal-derived DNA adduct by human Y-family DNA polymerases κ, τ, and Rev1 | - |
dc.type | Article | - |
dc.relation.issue | 38 | - |
dc.relation.volume | 49 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 8415 | - |
dc.relation.lastpage | 8424 | - |
dc.relation.journaltitle | Biochemistry | - |
dc.identifier.doi | 10.1021/bi1009024 | - |
dc.identifier.wosid | WOS:000281891500018 | - |
dc.identifier.scopusid | 2-s2.0-77957080930 | - |
dc.author.google | Maddukuri L. | - |
dc.author.google | Eoff R.L. | - |
dc.author.google | Choi J.-Y. | - |
dc.author.google | Rizzo C.J. | - |
dc.author.google | Guengerich F.P. | - |
dc.author.google | Marnett L.J. | - |
dc.contributor.scopusid | 최정윤(57223660142;34973862000) | - |
dc.date.modifydate | 20230627091252 | - |