View : 828 Download: 191
Serine 34 phosphorylation of Rho guanine dissociation inhibitor (RhoGDIα) links signaling from conventional protein kinase C to RhoGTPase in cell adhesion
- Title
- Serine 34 phosphorylation of Rho guanine dissociation inhibitor (RhoGDIα) links signaling from conventional protein kinase C to RhoGTPase in cell adhesion
- Authors
- Dovas A.; Choi Y.; Yoneda A.; Multhaupt H.A.B.; Kwon S.-H.; Kang D.; Oh E.-S.; Couchman J.R.
- Ewha Authors
- 오억수; 강동민
- SCOPUS Author ID
- 오억수
; 강동민
- Issue Date
- 2010
- Journal Title
- Journal of Biological Chemistry
- ISSN
- 0021-9258
- Citation
- Journal of Biological Chemistry vol. 285, no. 30, pp. 23296 - 23308
- Indexed
- SCI; SCIE; SCOPUS

- Document Type
- Article
- Abstract
- Conventional protein kinase C (PKC) isoforms are essential serine/threonine kinases regulating many signaling networks. At cell adhesion sites, PKCα can impact the actin cytoskeleton through its influence on RhoGTPases, but the intermediate steps are not well known. One important regulator of RhoGTPase function is the multifunctional guanine nucleotide dissociation inhibitor RhoGDIα that sequesters several related RhoGTPases in an inactive form, but it may also target them through interactions with actin-associated proteins. Here, it is demonstrated that conventional PKC phosphorylates RhoGDIα on serine 34, resulting in a specific decrease in affinity for RhoA but not Rac1 or Cdc42. The mechanism of RhoGDIα phosphorylation is distinct, requiring the kinase and phosphatidylinositol 4,5-bisphosphate, consistent with recent evidence that the inositide can activate, localize, and orient PKCα in membranes. Phosphospecific antibodies reveal endogenous phosphorylation in several cell types that is sensitive to adhesion events triggered, for example, by hepatocyte growth factor. Phosphorylation is also sensitive to PKC inhibition. Together with fluorescence resonance energy transfer microscopy sensing GTP-RhoA levels, the data reveal a common pathway in cell adhesion linking two essential mediators, conventional PKC and RhoA. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
- DOI
- 10.1074/jbc.M109.098129
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
-
Serine 34 phosphorylation of Rho guanine dissociation inhibitor (RhoGDIα) links signaling from conventional protein kinase C to RhoGTPase in cell adhesion.pdf(3.27 MB)
Download
- Export
- RIS (EndNote)
- XLS (Excel)
- XML