Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김희선 | * |
dc.date.accessioned | 2016-08-28T12:08:14Z | - |
dc.date.available | 2016-08-28T12:08:14Z | - |
dc.date.issued | 2010 | * |
dc.identifier.issn | 0009-2797 | * |
dc.identifier.other | OAK-6560 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220775 | - |
dc.description.abstract | Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that play a pivotal role in invasion and angiogenesis of malignant glioma cells. Therefore, the inhibition of MMPs has been suggested to be a promising therapeutic strategy for brain tumors. In the present study, we found that glycitein, a bacterial metabolite of the isoflavone glycitin, inhibits the expression of MMP-3 and MMP-9 at promoter, mRNA, and protein levels in PMA-stimulated U87MG human astroglioma cells. In addition, gelatin zymography showed that glycitein inhibited the PMA-induced MMP-9 secretion in U87MG cells. A subsequent Matrigel invasion assay revealed that glycitein suppresses the in vitro invasiveness of glioma cells, which may be at least partly due to the glycitein-mediated inhibition of MMP-3 and MMP-9. In support of this, treatment of MMP-3- or MMP-9-specific inhibitor significantly suppressed PMA-induced invasion of glioma cells. Further mechanistic studies revealed that glycitein inhibits the DNA binding and transcriptional activities of NF-κB and AP-1, which are important transcription factors for MMP-3 or MMP-9 gene expression. Furthermore, glycitein suppresses PMA-induced phosphorylation of three types of MAP kinases, which are upstream signaling molecules in MMP gene expressions and NF-κB and AP-1 activities in glioma cells. Therefore, the inhibition of MMP-3 and MMP-9 expression by glycitein may have therapeutic potential for controlling invasiveness of malignant gliomas. © 2010 Elsevier Ireland Ltd. | * |
dc.language | English | * |
dc.title | Glycitein inhibits glioma cell invasion through down-regulation of MMP-3 and MMP-9 gene expression | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 185 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 18 | * |
dc.relation.lastpage | 24 | * |
dc.relation.journaltitle | Chemico-Biological Interactions | * |
dc.identifier.doi | 10.1016/j.cbi.2010.02.037 | * |
dc.identifier.wosid | WOS:000277751300004 | * |
dc.identifier.scopusid | 2-s2.0-77950923898 | * |
dc.author.google | Lee E.-J. | * |
dc.author.google | Kim S.-Y. | * |
dc.author.google | Hyun J.-W. | * |
dc.author.google | Min S.-W. | * |
dc.author.google | Kim D.-H. | * |
dc.author.google | Kim H.-S. | * |
dc.contributor.scopusid | 김희선(57191372551) | * |
dc.date.modifydate | 20240118140922 | * |