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dc.contributor.author강동민-
dc.date.accessioned2016-08-28T12:08:45Z-
dc.date.available2016-08-28T12:08:45Z-
dc.date.issued2010-
dc.identifier.issn0743-7463-
dc.identifier.otherOAK-6228-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/220492-
dc.description.abstractA new chromogenic chemosensor based on lysine-functionalized silver nanoparticles 1 was prepared, and characterized by transmission electron microscopy (TEM), Fourier transform. Raman, and ultraviolet-visible (UV-vis) spectroscopy. The color changes of nanoparticles 1 in the absence and the presence of metal ion were observed, upon addition of various amino acids and proteins in aqueous solution. Among the various amino acids, the sensor 1 in the absence of metal ion shows a novel colorimetric sensor with capability to probe histidine and histidine-tagged proteins. On the other hand, the color changes of 1 in the presence of metal ions such as KCl or NiCl2 did not occur with any amino acids. Therefore, the sensor 1 in the absence of metal ion responds selectively to histidine, a response which can be attributed to its aggregation induced by histidine with high numbers of electrostatic interactions. This highly selective sensor 1 allows a rapid, quantitative assay of histidine to concentrations as low as 5.0 μM, providing a new tool for the direct measurement of histidine and histidine-tagged proteins in vitro system. Furthermore, we examined the effect of pH on absorbance (A520) of 1 in the presence of histidine (pH 4-12). The absorbance under basic conditions was higher than that under acidic or neutral conditions, in accord with the stronger aggregation of 1 with histidine by electrostatic interaction between the carboxylate anion of 1 and ammonium protons of histidine under basic conditions. © 2009 American Chemical Society.-
dc.languageEnglish-
dc.titleLysine-functionalized silver nanoparticles for visual detection and separation of histidine and histidine-tagged proteins-
dc.typeArticle-
dc.relation.issue3-
dc.relation.volume26-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage2181-
dc.relation.lastpage2185-
dc.relation.journaltitleLangmuir-
dc.identifier.doi10.1021/la9026865-
dc.identifier.wosidWOS:000273831700124-
dc.identifier.scopusid2-s2.0-75749147217-
dc.author.googleBae D.R.-
dc.author.googleHan W.S.-
dc.author.googleLim J.M.-
dc.author.googleKang S.-
dc.author.googleLee J.Y.-
dc.author.googleKang D.-
dc.author.googleJung J.H.-
dc.contributor.scopusid강동민(13103841000)-
dc.date.modifydate20190901081003-
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자연과학대학 > 생명과학전공 > Journal papers
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