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일반대학원
뇌·인지과학과
Journal papers
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Label-free fluorescent real-time monitoring of adenylyl cyclase
Title
Label-free fluorescent real-time monitoring of adenylyl cyclase
Authors
Rhee H.-W.
;
Kim K.-S.
;
Han P.-L.
;
Hong J.-I.
Ewha Authors
한평림
;
김경심
SCOPUS Author ID
한평림
Issue Date
2010
Journal Title
Bioorganic and Medicinal Chemistry Letters
ISSN
0960-894X
Citation
Bioorganic and Medicinal Chemistry Letters vol. 20, no. 3, pp. 1145 - 1147
Indexed
SCI; SCIE; SCOPUS
Document Type
Article
Abstract
In cellular signaling, adenylyl cyclase plays a key role in the hydrolysis of ATP to cyclic AMP and pyrophosphate. Using a synthetic fluorescent chemosensor (PyDPA) which binds strongly to the pyrophosphate group, we have developed a label-free fluorescent real-time detection system for adenylyl cyclase. This assay would be the first adenylyl cyclase assay based on chemosensing the production of pyrophosphate. © 2009 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.bmcl.2009.12.018
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