Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김태헌 | * |
dc.date.accessioned | 2016-08-28T12:08:44Z | - |
dc.date.available | 2016-08-28T12:08:44Z | - |
dc.date.issued | 2010 | * |
dc.identifier.issn | 1478-3223 | * |
dc.identifier.other | OAK-6218 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220483 | - |
dc.description.abstract | Background: MicroRNAs (miRNAs) have emerged as novel genetic regulators of cell functions such as proliferation, apoptosis and cancer. Aims: The aim of this study was to evaluate the role of a specific miRNA in modulating hepatic cell functions. Methods: C57Bl/6 mice were administered anti-fas receptor antibodies to induce liver cell apoptosis. miRNAs were purified from the liver tissue and evaluated using an miRNA microarray. The role of miRNA-491_5p, which was overexpressed in the model, in modulating hepatic cell functions was evaluated. miRNA-491_5p was overexpressed in Hep G2 cells, followed by the addition of tumour necrosis factor (TNF)-α, and induction of apoptosis as well as genes involved in apoptosis pathways were evaluated. The effect of miRNA-491_5p target genes on apoptosis was also analysed by inhibiting their expression by siRNA-induced gene silencing. Results: Upregulation of miRNA-491_5p was found in a high-dose anti-fas receptor antibody group. Overexpression of microRNA-491_5p sensitized Hep G2 cells for TNF-α-induced apoptosis, and also caused an inhibition of α-fetoprotein, (AFP), heat shock protein-90 (hsp-90) and nuclear factor-κB (NF-κB). Overexpression of miRNA-491_5p or inhibition of AFP and hsp-90 resulted in an increased apoptosis in TNF-α-treated Hep G2 cells. Conclusions: One of the miRNAs that is associated with the acute liver injury mouse model, miRNA-491_5p, sensitizes Hep G2 cells for TNF-α-induced apoptosis, at least in part, by inhibiting AFP, hsp-90 and NF-κB. © 2009 John Wiley & Sons A/S. | * |
dc.language | English | * |
dc.title | Acute liver injury upregulates microRNA-491-5p in mice, and its overexpression sensitizes Hep G2 cells for tumour necrosis factor-α-induced apoptosis | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 30 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 376 | * |
dc.relation.lastpage | 387 | * |
dc.relation.journaltitle | Liver International | * |
dc.identifier.doi | 10.1111/j.1478-3231.2009.02181.x | * |
dc.identifier.wosid | WOS:000273731500006 | * |
dc.identifier.scopusid | 2-s2.0-77950619689 | * |
dc.author.google | Yoon S. | * |
dc.author.google | Kim T.-H. | * |
dc.author.google | Natarajan A. | * |
dc.author.google | Wang S. | * |
dc.author.google | Choi J. | * |
dc.author.google | Wu J. | * |
dc.author.google | Zern M.A. | * |
dc.author.google | Venugopal S.K. | * |
dc.contributor.scopusid | 김태헌(57125156300;57219781484) | * |
dc.date.modifydate | 20240422114851 | * |