Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김명래 | * |
dc.contributor.author | 김선종 | * |
dc.date.accessioned | 2016-08-28T12:08:39Z | - |
dc.date.available | 2016-08-28T12:08:39Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 0513-5796 | * |
dc.identifier.other | OAK-6156 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220429 | - |
dc.description.abstract | Purpose: The aim of this study was to evaluate the survival, proliferation, and bone formation of dog mesenchymal stem cells (dMSCs) in the graft material by using Polycaprolactone-tricalcium phosphate (PCL-TCP), auto-fibrin glue (AFG), recombinant human bone morphogenetic protein-2 (rhBMP-2), and dMSCs after a transplantation to the scapula of adult beagle dogs. Materials and Methods: The subjects were two beagle dogs. Total dose of rhBMP-2 on each block was 10 μg with 50 μg/mg concentration. The cortical bone of the scapula of the dog was removed which was the same size of PCL-TCP block (Osteopore International Pte, Singapore; 5.0 x 5.0 x 8.0 mm in size), and the following graft material then was fixed with orthodontic mini-implant, Dual-top® (Titanium alloy, Jeil Co. Seoul, Korea). Four experimental groups were prepared for this study, Group 1: PCL-TCP + aFG; Group 2: PCL-TCP + aFG + dMSCs; Group 3: PCL-TCP + aFG + dMSCs + rhBMP-2; Group 4: PCL-TCP + aFG + dMSCs + rhBMP-2 + PCL membrane. The survival or proliferation of dMSCs cells was identified with an extracted tissue through a fluorescence microscope, H-E staining and Von-Kossa staining in two weeks and four weeks after the transplantation. Results: The survival and proliferation of dMSCs were identified through a fluorescence microscope from both Group 1 and Group 2 in two weeks and four weeks after the transplantation. Histological observation also found that the injected cells were proliferating well in the G2, G3, and G4 scaffolds. Conclusion: This study concluded that bone ingrowth occurred in PCL-TCP scaffold which was transplanted with rhBMP-2, and MSCs did not affect bone growth. More sufficient healing time would be needed to recognize effects of dMSCs on bone formation. © Copyright: Yonsei University College of Medicine 2009. | * |
dc.language | English | * |
dc.title | Effects of polycaprolactone-tricalcium phosphate, recombinant human bone morphogenetic protein-2 and dog mesenchymal stem cells on bone formation: Pilot study in dogs | * |
dc.type | Article | * |
dc.relation.issue | 6 | * |
dc.relation.volume | 50 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 825 | * |
dc.relation.lastpage | 831 | * |
dc.relation.journaltitle | Yonsei Medical Journal | * |
dc.identifier.doi | 10.3349/ymj.2009.50.6.825 | * |
dc.identifier.wosid | WOS:000272993700015 | * |
dc.identifier.scopusid | 2-s2.0-74549177537 | * |
dc.author.google | Kim S.-J. | * |
dc.author.google | Kim M.-R. | * |
dc.author.google | Oh J.-S. | * |
dc.author.google | Han I. | * |
dc.author.google | Shin S.-W. | * |
dc.contributor.scopusid | 김명래(55820385500) | * |
dc.contributor.scopusid | 김선종(55864004900) | * |
dc.date.modifydate | 20240130112828 | * |