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dc.contributor.author정병문*
dc.contributor.author김진흥*
dc.date.accessioned2016-08-28T12:08:20Z-
dc.date.available2016-08-28T12:08:20Z-
dc.date.issued2009*
dc.identifier.issn0141-0229*
dc.identifier.otherOAK-5896*
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/220242-
dc.description.abstractFructose-1,6-bisphosphate (FBP) aldolase, is a glycolytic enzyme that catalyzes the reversible condensation reaction of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P). The aldolase gene from Aquifex aeolicus was subcloned, overexpressed in E. coli and purified to 95% homogeneity. The purified enzyme was activated by high concentrations of NH4+ and low concentrations of Co2+. The native molecular weight of the purified FBP aldolase was identified as 67 kDa (dimer) by gel filtration chromatography. The enzyme exhibits optimum pH at 6.5 and temperature at 90 °C. Based on the kinetic characterizations, the apparent Km was calculated to be 4.4 ± 0.07 mM, while Vmax was found to be 100 ± 0.02 μM min-1 mg protein-1. The recombinant protein showed extreme heat stability; no activity loss was observed even at 100 °C for 2 h. In addition, the thermophilic enzyme also showed higher stability against several organic solvents viz. acetonitrile, 1,4-dioxane, and methanol. With higher stability against both heat and organic solvents than any other class II aldolase, the A. aeolicus FBP aldolase is an attractive enzyme for use as a biocatalyst for industrial applications. © 2009 Elsevier Inc. All rights reserved.*
dc.languageEnglish*
dc.titleCharacterization of a extreme thermostable fructose-1,6-bisphosphate aldolase from hyperthermophilic bacterium Aquifex aeolicus*
dc.typeArticle*
dc.relation.issue4*
dc.relation.volume45*
dc.relation.indexSCI*
dc.relation.indexSCIE*
dc.relation.indexSCOPUS*
dc.relation.startpage261*
dc.relation.lastpage266*
dc.relation.journaltitleEnzyme and Microbial Technology*
dc.identifier.doi10.1016/j.enzmictec.2009.06.012*
dc.identifier.wosidWOS:000270016100004*
dc.identifier.scopusid2-s2.0-68349157652*
dc.author.googleLa I.-J.*
dc.author.googleEum D.-Y.*
dc.author.googleGedi V.*
dc.author.googleKim J.*
dc.author.googleJeong B.*
dc.author.googleYoon M.-Y.*
dc.contributor.scopusid정병문(7102237959)*
dc.contributor.scopusid김진흥(8580015800)*
dc.date.modifydate20240118155902*
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자연과학대학 > 화학·나노과학전공 > Journal papers
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