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Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-κB activity
- Suppression of inducible nitric oxide synthase by (-)-isoeleutherin from the bulbs of Eleutherine americana through the regulation of NF-κB activity
- Song S.-H.; Min H.-Y.; Han A.-R.; Nam J.-W.; Seo E.-K.; Seoung Woo Park; Sang Hyung Lee; Sang Kook Lee
- Ewha Authors
- 이상국; 서은경; 한아름
- SCOPUS Author ID
- 이상국; 서은경; 한아름
- Issue Date
- Journal Title
- International Immunopharmacology
- International Immunopharmacology vol. 9, no. 3, pp. 298 - 302
- SCI; SCIE; SCOPUS
- Document Type
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- Eleutherine americana Merr. et Heyne (Iridaceae) has been used as a folk medicine for the treatment of coronary disorders and wound-healing. In our previous phytochemical study, several pyranonaphthoquinoids, including (-)-isoeleutherin, were isolated from the bulbs of E. americana. Because inhibitors of inducible nitric oxide synthase (iNOS) might be useful as anti-inflammatory agents, we investigated the potential of pyranonaphthoquinoids to inhibit iNOS activity. We found that (-)-isoeleutherin inhibits lipopolysaccharide (LPS)-stimulated induction of nitric oxide (NO) in a dose-dependent manner (IC50 = 7.4 μM). Using western blots and reverse transcription-polymerase chain reaction, we showed that (-)-isoeleutherin suppresses the expression of iNOS protein and mRNA. In addition, (-)-isoeleutherin inhibited the expression of various cytokines such as interleukin-1β and interferon-β. Activation of the transcriptional activity of NF-κB by LPS was also inhibited by treatment with (-)-isoeleutherin, suggesting that (-)-isoeleutherin-mediated suppression of iNOS expression is associated with the regulation of transcription factor NF-κB. These findings suggest that (-)-isoeleutherin might be a novel anti-inflammatory agent, and that it may work by inhibiting NFkB activation in macrophages. © 2008 Elsevier B.V. All rights reserved.
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