Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조경숙 | * |
dc.contributor.author | 이은희 | * |
dc.date.accessioned | 2016-08-28T12:08:09Z | - |
dc.date.available | 2016-08-28T12:08:09Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 0254-8704 | * |
dc.identifier.other | OAK-5348 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220128 | - |
dc.description.abstract | Rhodococcus sp. EH831 is a microbial species that can degrade volatile organic compounds. We optimized a method for monitoring quantitative real-time PCR (qRT-PCR) of EH831 that was incorporated into a polyurethane (PU) biofilter. When the genomic DNA of EH831 was directly extracted from a PU sample with immobilized EH831, the recovery efficiency was very low due to DNA absorption into the PU. DNA amplification during PCR was also inhibited by PU impurities. Therefore, a pre-treatment step was necessary. We successfully recovered cells from the PU by squeezing the matrix, adding sterilized water, and vortexing. The recovery efficiency ranged from 105 to 144%, and there was no statistically significant difference. We designed a novel TaqMan probe for EH831 and demonstrated its high specificity for EH831. The detection range for EH831 was 105-1011 CFU ml-1. The method described in this study can be used to investigate the relationship between quantitative analysis of Rhodococcus sp. EH831 and PU biofilter performance. © Triveni Enterprises, Lucknow (India). | * |
dc.language | English | * |
dc.title | Application of quantitative real-time PCR for quantification of Rhodococcus sp. EH831 in a polyurethane biofilter | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 30 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 155 | * |
dc.relation.lastpage | 159 | * |
dc.relation.journaltitle | Journal of Environmental Biology | * |
dc.identifier.wosid | WOS:000262706900025 | * |
dc.identifier.scopusid | 2-s2.0-59349102118 | * |
dc.author.google | Lee E.H. | * |
dc.author.google | Cho K.S. | * |
dc.author.google | Ryu H.W. | * |
dc.contributor.scopusid | 조경숙(7403957095) | * |
dc.contributor.scopusid | 이은희(55773710100;56141882500) | * |
dc.date.modifydate | 20240322131338 | * |