Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 서석효 | * |
dc.contributor.author | 최신규 | * |
dc.contributor.author | 박성희 | * |
dc.date.accessioned | 2016-08-28T12:08:03Z | - |
dc.date.available | 2016-08-28T12:08:03Z | - |
dc.date.issued | 2008 | * |
dc.identifier.issn | 0031-6768 | * |
dc.identifier.other | OAK-5071 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220064 | - |
dc.description.abstract | We studied the effects of Na+ influx on large-conductance Ca2+-activated K+ (BKCa) channels in cultured human umbilical vein endothelial cells (HUVECs) by means of patch clamp and SBFI microfluorescence measurements. In current-clamped HUVECs, extracellular Na+ replacement by NMDG+ or mannitol hyperpolarized cells. In voltage-clamped HUVECs, changing membrane potential from 0 mV to negative potentials increased intracellular Na+ concentration ([Na +]i) and vice versa. In addition, extracellular Na + depletion decreased [Na+]i. In voltage-clamped cells, BKCa currents were markedly increased by extracellular Na+ depletion. In inside-out patches, increasing [Na+]i from 0 to 20 or 40 mM reduced single channel conductance but not open probability (NPo) of BKCa channels and decreasing intracellular K+ concentration ([K+] i) gradually from 140 to 70 mM reduced both single channel conductance and NPo. Furthermore, increasing [Na+]i gradually from 0 to 70 mM, by replacing K+, markedly reduced single channel conductance and NPo. The Na+-Ca2+ exchange blocker Ni2+ or KB-R7943 decreased [Na+]i and increased BKCa currents simultaneously, and the Na+ ionophore monensin completely inhibited BKCa currents. BK Ca currents were significantly augmented by increasing extracellular K+ concentration ([K+]o) from 6 to 12 mM and significantly reduced by decreasing [K+]o from 12 or 6 to 0 mM or applying the Na+-K+ pump inhibitor ouabain. These results suggest that intracellular Na+ inhibit single channel conductance of BKCa channels and that intracellular K+ increases single channel conductance and NPo. © 2008 Springer-Verlag. | * |
dc.language | English | * |
dc.title | Intracellular Na+ modulates large conductance Ca 2+-activated K+ currents in human umbilical vein endothelial cells | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 457 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 67 | * |
dc.relation.lastpage | 75 | * |
dc.relation.journaltitle | Pflugers Archiv European Journal of Physiology | * |
dc.identifier.doi | 10.1007/s00424-008-0490-9 | * |
dc.identifier.wosid | WOS:000259373000007 | * |
dc.identifier.scopusid | 2-s2.0-52549122069 | * |
dc.author.google | Liang G.H. | * |
dc.author.google | Kim M.Y. | * |
dc.author.google | Park S. | * |
dc.author.google | Kim J.A. | * |
dc.author.google | Choi S. | * |
dc.author.google | Suh S.H. | * |
dc.contributor.scopusid | 서석효(55666113100) | * |
dc.contributor.scopusid | 최신규(12783276200) | * |
dc.contributor.scopusid | 박성희(8848996000) | * |
dc.date.modifydate | 20240220100710 | * |