Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조용재 | * |
dc.date.accessioned | 2016-08-28T12:08:56Z | - |
dc.date.available | 2016-08-28T12:08:56Z | - |
dc.date.issued | 2008 | * |
dc.identifier.issn | 0513-5796 | * |
dc.identifier.other | OAK-4782 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219997 | - |
dc.description.abstract | Purpose: This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization. Materials and Methods: Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the Genbank™ database. Results: Using semi-quantitative reverse transription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line. Conclusion: Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed. | * |
dc.language | English | * |
dc.title | Identification of up-regulated genes in malignant glioma with subtraction hybridization: Preliminary screening studies | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 49 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 301 | * |
dc.relation.lastpage | 310 | * |
dc.relation.journaltitle | Yonsei Medical Journal | * |
dc.identifier.doi | 10.3349/ymj.2008.49.2.301 | * |
dc.identifier.wosid | WOS:000255521500018 | * |
dc.identifier.scopusid | 2-s2.0-44649119458 | * |
dc.author.google | Cho Y.J. | * |
dc.contributor.scopusid | 조용재(55472207600) | * |
dc.date.modifydate | 20240422113935 | * |