Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2016-08-28T12:08:53Z | - |
dc.date.available | 2016-08-28T12:08:53Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.other | OAK-4679 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219969 | - |
dc.description.abstract | Two sugar biosynthetic cassette plasmids were used to direct the biosynthesis of a deoxyaminosugar. The pOTBP1 plasmid containing TDP-glucose synthase (desIII), TDP-glucose4, 6-dehydratase (desIV), and glycosyltransferase (desVII/desVIII) was constructed and transformed into S. venezuelae YJ003, a strain in which the entire gene cluster of desosamine biosynthesis is deleted. The expression plasmid pOTBP3 containing 4-aminotransferase (gerB) and 3,5-epimerase (orf9) was transformed again into S. venezuelae YJ003-OTBP1 to obtain S. venezuelue YJ003-OTBP3 for the production of 4-amino-4,6-dideoxy-L-glucose derivatives. The crude extracts obtained from S. venezuelae ATCC 15439, S. venezuelae YJ003, and S. venezuelae YJ003-OTBP3 were further analyzed by TLC, bioassay, HPLC, ESI/MS, LC/MS, and MS/MS. The results of our study clearly shows that S. venezuelae YJ003-OTBP3 constructs other new hybrid macrolide derivatives including 4-amino-4,6-dideoxy-L-glycosylated YC-17 (3, [M+ Na+ m/z= 464.5), methymycin (4, m/z=480.5), novamethymycin (6, m/z=496.5), and pikromycin (5, m/z=536.5) from a 12-membered ring aglycon (10-deoxymethynolide, 1) and a 14-membered ring aglycon (narbonolide, 2). These results suggest a successful engineering of a deoxysugar pathway to generate novel hybrid macrolide derivatives, including deoxyaminosugar. © The Korean Society for Microbiology and Biotechnology. | - |
dc.language | English | - |
dc.title | Genetically engineered biosynthesis of macrolide derivatives including 4-amino-4,6-dideoxy-L-glucose from Streptomyces venezuelae YJ003-OTBP3 | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 18 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 88 | - |
dc.relation.lastpage | 94 | - |
dc.relation.journaltitle | Journal of Microbiology and Biotechnology | - |
dc.identifier.wosid | WOS:000254047600014 | - |
dc.identifier.scopusid | 2-s2.0-43749107330 | - |
dc.author.google | Pageni B.B. | - |
dc.author.google | Oh T.-J. | - |
dc.author.google | Liou K.K. | - |
dc.author.google | Yoon Y.J. | - |
dc.author.google | Sohng J.K. | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210708161345 | - |