View : 609 Download: 190
LIME, a Novel Transmembrane Adaptor Protein, Associates with p56 lck and Mediates T Cell Activation
- Title
- LIME, a Novel Transmembrane Adaptor Protein, Associates with p56 lck and Mediates T Cell Activation
- Authors
- Hur E.M.; Son M.; Lee O.-H.; Choi Y.B.; Park C.; Lee H.; Yun Y.
- Ewha Authors
- 윤영대
- SCOPUS Author ID
- 윤영대
- Issue Date
- 2003
- Journal Title
- Journal of Experimental Medicine
- ISSN
- 0022-1007
- Citation
- Journal of Experimental Medicine vol. 198, no. 10, pp. 1463 - 1473
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- In this study, we identify and characterize a novel transmembrane adaptor protein, designated Lck-interacting membrane protein (LIME), as a binding partner of the Lck Src homology (SH)2 domain. LIME possesses a short extracellular domain, a transmembrane domain, and a cytoplasmic tail containing five tyrosine-based motifs. The protein is primarily expressed in hematopoietic cells and lung. Interestingly, LIME expression is up-regulated by TCR stimulation and sustained up to 24 h, suggesting that LIME acts throughout the early to late stages of T cell activation. LIME is localized to membrane rafts and distributed within the T cell-APC contact site. Upon TCR stimulation of Jurkat T cells, LIME associates with Lck as a tyrosine-phosphorylated protein. Experiments using Jurkat T cells expressing CD8-LIME chimera reveal that the protein associates with phosphatidylinositol 3-kinase, Grb2, Gads, and SHP2, and activates ERK1/2 and JNK but not p38. Moreover, overexpression of LIME in Jurkat T cells induces transcriptional activation of the IL-2 promoter. Our data collectively show that LIME is a raft-associated transmembrane adaptor protein linking TCR stimuli to downstream signaling pathways via associations with Lck.
- DOI
- 10.1084/jem.20030232
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
-
LIME, a Novel Transmembrane Adaptor Protein, Associates with p56 lck and Mediates T Cell Activation.pdf(319.74 kB)
Download
- Export
- RIS (EndNote)
- XLS (Excel)
- XML