Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 신윤용 | - |
dc.date.accessioned | 2016-08-28T11:08:30Z | - |
dc.date.available | 2016-08-28T11:08:30Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0022-3565 | - |
dc.identifier.other | OAK-1684 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219322 | - |
dc.description.abstract | Peroxisome proliferator-activated receptor-γ (PPAR-γ) ligands have been demonstrated to inhibit growth of several cancer cells. Here, we investigated whether one of the PPAR-γ ligands, 15-deoxy-Δ 12,14-prostaglandin J2 (15-deoxy-PGJ 2) inhibits cell growth of two human neuroblastoma cells (SK-N-SH and SK-N-MC) in a PPAR-γ-dependent manner. PPAR-γ was expressed in these cells, and 15-deoxy-PGJ 2 increased expression, DNA binding activity, and transcriptional activity of PPAR-γ. 15-Deoxy-PGJ 2 also inhibited cell growth in time- and dose-dependent manners in both cells. Cells were arrested in G 2/M phase after 15-deoxy-PGJ 2 treatment with concomitant increase in the expression of G 2/M phase regulatory protein cyclin B1 but decrease in the expression of cdk2, cdk4, cyclin A, cyclin D1, cyclin E, and cdc25C. Conversely, related to the growth inhibitory effect, 15-deoxy-PGJ 2 increased the induction of apoptosis in a dose-dependent manner. Consistent with the induction of apoptosis, 15-deoxy-PGJ 2 increased the expression of proapoptotic proteins caspase 3, caspase 9, and Bax but down-regulated antiapoptotic protein Bcl-2. 15-Deoxy-PGJ 2 also activated extracellular signal-regulated kinase (ERK) 2. In addition, mitogen-activated protein kinase kinase (MEK) 1/2 inhibitor PD98059 (2′-amino-3′-methoxyflavone) decreased 15-deoxy-PGJ 2-induced ERK2 activation, and expression of PPAR-γ, capase-3, and cyclin B1. Moreover, MEK1/2 inhibitor PD98059 significantly prevented against the 15-deoxy-PGJ 2-induced cell growth inhibition. We also found that PPAR-γ antagonist GW9662 (2-chloro-5-nitro-N-phenylbenzamide) reversed the 15-deoxy-PGJ 2-induced cell growth inhibition, PPAR-γ expression, and activation of ERK2. These results demonstrate that 15-deoxy-PGJ 2 inhibits growth of human neuroblastoma cells via the induction of apoptosis in a PPAR-γ-dependent manner through activation of ERK pathway and suggest that 15-deoxy-PGJ 2 may have promising application as a therapeutic agent for neuroblastoma. | - |
dc.language | English | - |
dc.title | Peroxisome Proliferator-Activated Receptor-γ Activator 15-Deoxy-Δ 12,14-Prostaglandin J 2 Inhibits Neuroblastoma Cell Growth through Induction of Apoptosis: Association with Extracellular Signal-Regulated Kinase Signal Pathway | - |
dc.type | Article | - |
dc.relation.issue | 2 | - |
dc.relation.volume | 307 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 505 | - |
dc.relation.lastpage | 517 | - |
dc.relation.journaltitle | Journal of Pharmacology and Experimental Therapeutics | - |
dc.identifier.doi | 10.1124/jpet.103.053876 | - |
dc.identifier.wosid | WOS:000186076600010 | - |
dc.identifier.scopusid | 2-s2.0-0142210246 | - |
dc.author.google | Kim E.J. | - |
dc.author.google | Park K.S. | - |
dc.author.google | Chung S.Y. | - |
dc.author.google | Sheen Y.Y. | - |
dc.author.google | Moon D.C. | - |
dc.author.google | Song Y.S. | - |
dc.author.google | Kim K.S. | - |
dc.author.google | Song S. | - |
dc.author.google | Yun Y.P. | - |
dc.author.google | Lee M.K. | - |
dc.author.google | Oh K.W. | - |
dc.author.google | Yoon D.Y. | - |
dc.author.google | Hong J.T. | - |
dc.contributor.scopusid | 신윤용(6603872711) | - |
dc.date.modifydate | 20230411104830 | - |