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dc.contributor.author박혜영-
dc.contributor.author한후재-
dc.contributor.author전루민-
dc.date.accessioned2016-08-28T11:08:45Z-
dc.date.available2016-08-28T11:08:45Z-
dc.date.issued2001-
dc.identifier.issn0271-3683-
dc.identifier.otherOAK-790-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/218841-
dc.description.abstractPurpose. By using bothin vivo andin vitro (organ-cultured) systems, the optimal concentrations of hEGF to enhance epithelial healing after alkali wounds were evaluated in the rabbit cornea. Methods. Alkali-injured corneas (φ = 5.5 mm, 1 N NaOH, 60 s) were treated with 0.01, 0.1, 1.0, 10 and 100 ng/ml hEGF for thein vitro study. The healing of epithelium and endothelium was determined at 1, 2, 3, 4, and 6 days after treatment. For thein vivo experiment, the eyes were treated with 2, 5, 10, and 50 mg/ml hEGF 3 times per day. The measurement of epithelial healing rate, transmission electron microscopy and immunohistochemical observation were performed after 7 days treatment. Results. In in vitro tests, hEGF enhanced the epithelial healing rates, showing a maximum enhancement at the concentration of 1.0 ng/ml, and endothelial healing was increased at 100 ng/ml. In in vivo studies, no significant difference was observed in the rates of epithelial healing between control and each hEGF-treated group. Among the tested concentrations, 5 μg/ml hEGF induced the most active proliferation of basal cells and 50 μg/ml hEGF remarkably produced a vascular ingrowth to the central wound area. The thickness of re-surfaced epithelium was increased by hEGF in a concentration-dependent manner. Conclusions. The results of the present study indicate that a low concentration of hEGF may selectively enhance epithelial healing without affecting endothelial healing. The optimal concentration of hEGF for the stimulation of epithelial healing appears to be 5 μg/ml in rabbit corneal alkali wounds.-
dc.languageEnglish-
dc.titleOptimal concentration of human epidermal growth factor (hEGF) for epithelial healing in experimental corneal alkali wounds-
dc.typeArticle-
dc.relation.issue4-
dc.relation.volume22-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage272-
dc.relation.lastpage279-
dc.relation.journaltitleCurrent Eye Research-
dc.identifier.doi10.1076/ceyr.22.4.272.5507-
dc.identifier.wosidWOS:000170203600005-
dc.identifier.scopusid2-s2.0-0034821687-
dc.author.googleKim M.-J.-
dc.author.googleJun R.-M.-
dc.author.googleKim W.-K.-
dc.author.googleHann H.-J.-
dc.author.googleChong Y.H.-
dc.author.googlePark H.-Y.-
dc.author.googleChung J.-H.-
dc.contributor.scopusid박혜영(7601567979)-
dc.contributor.scopusid한후재(8698607500)-
dc.contributor.scopusid전루민(6602278519)-
dc.date.modifydate20180329110106-
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의과대학 > 의학과 > Journal papers
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