Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박혜영 | * |
dc.contributor.author | 한후재 | * |
dc.contributor.author | 전루민 | * |
dc.date.accessioned | 2016-08-28T11:08:45Z | - |
dc.date.available | 2016-08-28T11:08:45Z | - |
dc.date.issued | 2001 | * |
dc.identifier.issn | 0271-3683 | * |
dc.identifier.other | OAK-790 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218841 | - |
dc.description.abstract | Purpose. By using bothin vivo andin vitro (organ-cultured) systems, the optimal concentrations of hEGF to enhance epithelial healing after alkali wounds were evaluated in the rabbit cornea. Methods. Alkali-injured corneas (φ = 5.5 mm, 1 N NaOH, 60 s) were treated with 0.01, 0.1, 1.0, 10 and 100 ng/ml hEGF for thein vitro study. The healing of epithelium and endothelium was determined at 1, 2, 3, 4, and 6 days after treatment. For thein vivo experiment, the eyes were treated with 2, 5, 10, and 50 mg/ml hEGF 3 times per day. The measurement of epithelial healing rate, transmission electron microscopy and immunohistochemical observation were performed after 7 days treatment. Results. In in vitro tests, hEGF enhanced the epithelial healing rates, showing a maximum enhancement at the concentration of 1.0 ng/ml, and endothelial healing was increased at 100 ng/ml. In in vivo studies, no significant difference was observed in the rates of epithelial healing between control and each hEGF-treated group. Among the tested concentrations, 5 μg/ml hEGF induced the most active proliferation of basal cells and 50 μg/ml hEGF remarkably produced a vascular ingrowth to the central wound area. The thickness of re-surfaced epithelium was increased by hEGF in a concentration-dependent manner. Conclusions. The results of the present study indicate that a low concentration of hEGF may selectively enhance epithelial healing without affecting endothelial healing. The optimal concentration of hEGF for the stimulation of epithelial healing appears to be 5 μg/ml in rabbit corneal alkali wounds. | * |
dc.language | English | * |
dc.title | Optimal concentration of human epidermal growth factor (hEGF) for epithelial healing in experimental corneal alkali wounds | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 22 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 272 | * |
dc.relation.lastpage | 279 | * |
dc.relation.journaltitle | Current Eye Research | * |
dc.identifier.doi | 10.1076/ceyr.22.4.272.5507 | * |
dc.identifier.wosid | WOS:000170203600005 | * |
dc.identifier.scopusid | 2-s2.0-0034821687 | * |
dc.author.google | Kim M.-J. | * |
dc.author.google | Jun R.-M. | * |
dc.author.google | Kim W.-K. | * |
dc.author.google | Hann H.-J. | * |
dc.author.google | Chong Y.H. | * |
dc.author.google | Park H.-Y. | * |
dc.author.google | Chung J.-H. | * |
dc.contributor.scopusid | 박혜영(7601567979) | * |
dc.contributor.scopusid | 한후재(8698607500) | * |
dc.contributor.scopusid | 전루민(6602278519) | * |
dc.date.modifydate | 20240422130207 | * |