View : 564 Download: 0
Caspase-cleaved TRAF1 negatively regulates the antiapoptotic signals of TRAF2 during TNF-induced cell death
- Title
- Caspase-cleaved TRAF1 negatively regulates the antiapoptotic signals of TRAF2 during TNF-induced cell death
- Authors
- Hyun Duk Jang; Young Mee Chung; Ji Hyun Baik; Young Geum Choi; Park I.-S.; Yong Keun Jung; Soo Young Lee
- Ewha Authors
- 이수영
- SCOPUS Author ID
- 이수영
- Issue Date
- 2001
- Journal Title
- Biochemical and Biophysical Research Communications
- ISSN
- 0006-291X
- Citation
- Biochemical and Biophysical Research Communications vol. 281, no. 2, pp. 499 - 505
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Tumor necrosis factor (TNF) signaling leads to pleiotropic responses in a wide range of cell types, in part by activating antiapoptotic and proapoptotic pathways. Previous studies have suggested that TNF receptor-associated factor (TRAF) 2 can mediate crucial antiapoptotic signals during TNF stimulation. However, it is unclear how the antiapoptotic signals via TRAF2 in TNF-R1 signaling is regulated. Here we show that TRAF1 is cleaved by caspase-8 into two fragments during apoptosis induced by TNF. Overexpression of the C-terminal cleavage product, TRAF1-c, increased TNF-induced cell death of hybridoma T cells. Importantly, we demonstrate that the cleavage product of TRAF1 coimmunoprecipitates with TRAF2 that is released from the TNF-R1 complex in response to prolonged TNF treatment. These results indicate that caspase-dependent cleavage of TRAF1 generates TRAF1-c fragments that are able to bind TRAF2, and then sequester TRAF2 from the TNF-R1 complex, rendering cells, at least in part, sensitive to TNF. © 2001 Academic Press.
- DOI
- 10.1006/bbrc.2001.4369
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML