View : 604 Download: 0

Influence of the antibody purification method on immunoassay performance: Hapten-antibody binding in accordance with the structure of the affinity column ligand

Title
Influence of the antibody purification method on immunoassay performance: Hapten-antibody binding in accordance with the structure of the affinity column ligand
Authors
Choi J.Kim C.Choi M.J.
Ewha Authors
김춘미
SCOPUS Author ID
김춘미scopus
Issue Date
1999
Journal Title
Analytical Biochemistry
ISSN
0003-2697JCR Link
Citation
Analytical Biochemistry vol. 274, no. 1, pp. 118 - 124
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
The effects of ligands for immunoaffinity chromatography on the immunoassay were investigated with three goat anti-methamphetamine (anti-MA) antibodies (Abs). An N-4-aminobutyl derivative of methamphetamine (4-ABMA) was conjugated with proteins and used as immunogens. All the antisera produced were purified by affinity chromatography with various ligands of 4- ABMA-proteins and of haptens as well as protein G: 4-ABMA-bovine serum albumin (4-ABMA-BSA), 4-ABMA-keyhole limpet hemocyanine (4-ABMA-KLH), 4-ABMA- ovalbumin (4-ABMA-OVA), MA, 4-ABMA, and amphetamine were used as ligands. Enzyme-linked immunosorbent assay (ELISA) was conducted to examine characteristics of the purified Abs with the 4-ABMA-OVA competitor coated. The results obtained revealed that characters of the purified Abs were closely related with chemical structures of ligands used. The Abs from the MA and the amphetamine columns showed better sensitivities than those from the others in each antiserum. Particularly, the Ab from the amphetamine column gave the best resuits in terms of sensitivity and specificity. The recognition or the affinity of the Ab selected was considered to be affected by the structure of the ligand concerned. These results suggest that the Ab purification method should be considered as an important parameter which has great influence on the performance of immunoassays with polyclonal Abs.
DOI
10.1006/abio.1999.4261
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

BROWSE