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A Selective Imidazoline-2-thione-Bearing Two-Photon Fluorescent Probe for Hypochlorous Acid in Mitochondria
- A Selective Imidazoline-2-thione-Bearing Two-Photon Fluorescent Probe for Hypochlorous Acid in Mitochondria
- Xu, Qingling; Heo, Cheol Ho; Kim, Jin A.; Lee, Hye Sue; Hu, Ying; Kim, Dayoung; Swamy, Kunemadihalli Mathada Kotraiah; Kim, Gyoungmi; Nam, Sang-Jip; Kim, Hwan Myung; Yoon, Juyoung
- Ewha Authors
- 윤주영; 남상집
- SCOPUS Author ID
- 윤주영; 남상집
- Issue Date
- Journal Title
- ANALYTICAL CHEMISTRY
- ANALYTICAL CHEMISTRY vol. 88, no. 12, pp. 6615 - 6620
- AMER CHEMICAL SOC
- SCIE; SCOPUS
- Document Type
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- Hypochlorite (OCl-) plays a key role in the immune system and is involved in various diseases. Accordingly, direct detection of endogenous OCl- at the subcellular level is important for understanding inflammation and cellular apoptosis. In the current study, a two-photon fluorescent off/on probe (PNIS) bearing imidazoline-2-thione as an OCl- recognition unit and triphenylphosphine (TPP) as a mitochondrial-targeting group was synthesized and examined for its ability to image mitochondrial OCl- in situ. This probe, based on the specific reaction between imidazoline-2-thione and OCl-, displayed a selective fluorescent off/on response to OCl- with the various reactive oxygen species in a physiological medium. PNIS was successfully applied to image of endogenously produced mitochondrial OCl- in live RAW 264.7 cells-via two-photon microscopy.
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