Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 권영주 | * |
dc.date.accessioned | 2016-08-28T11:08:50Z | - |
dc.date.available | 2016-08-28T11:08:50Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1949-2553 | * |
dc.identifier.other | OAK-18810 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218286 | - |
dc.description.abstract | Proteomic analysis of ionomycin-treated and untreated mammary epithelial MCF10A cells elucidated differences in Ku80 cleavage. Ku80, a subunit of the Ku protein complex, is an initiator of the non-homologous, end-joining (NHEJ), double-strand breaks (DSBs) repair pathway. The nuclear Ku80 was cleaved in a calcium concentration-dependent manner by m-calpain but not by m-calpain. The cleavage of nuclear Ku80 at its beta/chi{ domain was validated by Western blotting analysis using flag-tagged expression vectors of truncated versions of Ku80 and a flag antibody and was confirmed in m-calpain knock-down cells and in vitro cell-free evaluation with recombinant proteins of calpains, Ku70, and Ku80. In addition, the cleaved Ku80 still formed a Ku heterodimer and promoted DNA DSB repair activity. Taken together, these findings indicate that translocated m-calpain enhances the NHEJ pathway through the cleavage of Ku80. Based on the present study, m-calpain in DNA repair pathways might be a novel anticancer drug target, or its mechanism might be a possible route for resistance acquisition of DNA damage-inducing chemotherapeutics. | * |
dc.language | English | * |
dc.publisher | IMPACT JOURNALS LLC | * |
dc.subject | m-calpain | * |
dc.subject | Ku80-related DNA repair | * |
dc.subject | adriamycin | * |
dc.subject | DNA damage | * |
dc.title | Calcium influx-mediated translocation of m-calpain induces Ku80 cleavage and enhances the Ku80-related DNA repair pathway | * |
dc.type | Article | * |
dc.relation.issue | 21 | * |
dc.relation.volume | 7 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 30831 | * |
dc.relation.lastpage | 30844 | * |
dc.relation.journaltitle | ONCOTARGET | * |
dc.identifier.doi | 10.18632/oncotarget.8791 | * |
dc.identifier.wosid | WOS:000377746600075 | * |
dc.identifier.scopusid | 2-s2.0-84971577968 | * |
dc.author.google | Baek, Kyung Hye | * |
dc.author.google | Yu, Han Vit | * |
dc.author.google | Kim, Eosu | * |
dc.author.google | Na, Younghwa | * |
dc.author.google | Kwon, Youngjoo | * |
dc.contributor.scopusid | 권영주(12446435600) | * |
dc.date.modifydate | 20240422124907 | * |