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Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling

Title
Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling
Authors
Xie, KeqiangMasuho, IkuoShih, Chien-ChengCao, YanSasaki, KeitaLai, Chun Wan J.Han, Pyung-LimUeda, HiroshiDessauer, Carmen W.Ehrlich, Michelle E.Xu, BaojiWillardson, Barry M.Martemyanov, Kirill A.
Ewha Authors
한평림
SCOPUS Author ID
한평림scopus
Issue Date
2015
Journal Title
ELIFE
ISSN
2050-084XJCR Link
Citation
ELIFE vol. 4
Publisher
ELIFE SCIENCES PUBLICATIONS LTD
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
In the striatum, signaling via G protein-coupled neurotransmitter receptors is essential for motor control. Critical to this process is the effector enzyme adenylyl cyclase type 5 (AC5) that produces second messenger cAMP upon receptor-mediated activation by G protein Golf. However, the molecular organization of the G(olf)-AC5 signaling axis is not well understood. In this study, we report that in the striatum AC5 exists in a stable pre-coupled complex with subunits of G(olf) heterotrimer. We use genetic mouse models with disruption in individual components of the complex to reveal hierarchical order of interactions required for AC5-G(olf) stability. We further identify that the assembly of AC5-G(olf) complex is mediated by PhLP1 chaperone that plays central role in neurotransmitter receptor coupling to cAMP production motor learning. These findings provide evidence for the existence of stable G protein-effector signaling complexes and identify a new component essential for their assembly.
DOI
10.7554/eLife.10451
Appears in Collections:
일반대학원 > 뇌·인지과학과 > Journal papers
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