Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 조인호 | * |
dc.contributor.author | 박윤신 | * |
dc.contributor.author | 이지영 | * |
dc.date.accessioned | 2016-08-27T04:08:39Z | - |
dc.date.available | 2016-08-27T04:08:39Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 0006-291X | * |
dc.identifier.issn | 1090-2104 | * |
dc.identifier.other | OAK-16152 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217900 | - |
dc.description.abstract | We previously reported that hypoxia increases angiopoietin-1 (Ang1), but not Ang2, mRNA expression in bovine retinal pericytes (BRP). However, the mechanism underlying Ang1 expression is unknown. Here, we report that Ang1 protein expression increased in hypoxic BRP in a dose- and time-dependent manner. This increase was accompanied by an increase in hypoxia-inducible factor-2 alpha (HIF2 alpha) expression. Transfection with an antisense oligonucleotide for HIF2 alpha partially inhibited the hypoxia-induced increase in Ang1 expression. HIF2 alpha overexpression further potentiated hypoxia-stimulated Ang1 expression, suggesting that HIF2 alpha plays an important role in Ang1 regulation in BRP. When fused the Ang1 promoter (-3040 to +199) with the luciferase reporter gene, we found that hypoxia significantly increased promoter activity by 4.02 +/- 1.68 fold. However, progressive 5'-deletions from 3040 to 1799, which deleted two putative hypoxia response elements (HRE), abolished the hypoxia-induced increase in promoter activity. An electrophoretic mobility shift assay revealed that HIF2 alpha was predominantly bound to a HRE site, located specifically at nucleotides -2715 to -2712. Finally, treatment with conditioned medium obtained from hypoxic pericytes stimulated endothelial cell migration and tube formation, which was completely blocked by co-treatment with anti-Ang1 antibody. This study is the first to demonstrate that hypoxia upregulates Ang1 expression via HIF2 alpha-mediated transcriptional activation in pericytes, which plays a key role in angiogenesis. (C) 2015 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | * |
dc.subject | Angiogenesis | * |
dc.subject | Angiopoietin-1 | * |
dc.subject | Hypoxia-inducible factor-2 alpha | * |
dc.subject | Hypoxia | * |
dc.subject | Pericytes | * |
dc.subject | Endothelial cells | * |
dc.title | Expression of angiopoietin-1 in hypoxic pericytes: Regulation by hypoxia-inducible factor-2 alpha and participation in endothelial cell migration and tube formation | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 469 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 263 | * |
dc.relation.lastpage | 269 | * |
dc.relation.journaltitle | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | * |
dc.identifier.doi | 10.1016/j.bbrc.2015.11.108 | * |
dc.identifier.wosid | WOS:000368652300019 | * |
dc.identifier.scopusid | 2-s2.0-84951010640 | * |
dc.author.google | Park, Yoon Shin | * |
dc.author.google | Kim, Gyungah | * |
dc.author.google | Jin, Yoon Mi | * |
dc.author.google | Lee, Jee Young | * |
dc.author.google | Shin, Jong Wook | * |
dc.author.google | Jo, Inho | * |
dc.contributor.scopusid | 조인호(26643129000;56663841900) | * |
dc.contributor.scopusid | 박윤신(35975370400) | * |
dc.contributor.scopusid | 이지영(55540732300;57212925259) | * |
dc.date.modifydate | 20240222162940 | * |