Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 신윤용 | * |
dc.contributor.author | 김대기 | * |
dc.contributor.author | 임우성 | * |
dc.date.accessioned | 2016-08-27T04:08:39Z | - |
dc.date.available | 2016-08-27T04:08:39Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 1949-2553 | * |
dc.identifier.other | OAK-16136 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217897 | - |
dc.description.abstract | Distant relapse after chemotherapy is an important clinical issue for treating breast cancer patients and results from the development of cancer stem-like cells (CSCs) during chemotherapy. Here we report that blocking epithelial-to-mesenchymal transition (EMT) suppresses paclitaxel-induced CSCs properties by using a MDA-MB-231-xenografted mice model (in vivo), and breast cancer cell lines (in vitro). Paclitaxel, one of the cytotoxic taxane-drugs such as docetaxel, increases mesenchymal markers (Vimentin and Fibronectin) and decreases an epithelial marker (Zo-1). Blocking TGF-beta signaling with the TGF-beta type I receptor kinase (ALK5) inhibitor, EW-7197, suppresses paclitaxel-induced EMT and CSC properties such as mammosphere-forming efficiency (MSFE), aldehyde dehydrogenase (ALDH) activity, CD44(+)/CD24(-) ratio, and pluripotency regulators (Oct4, Nanog, Klf4, Myc, and Sox2). The combinatorial treatment of EW-7197 improves the therapeutic effect of paclitaxel by decreasing the lung metastasis and increasing the survival time in vivo. We confirmed that Snail is increased by paclitaxel-induced intracellular reactive oxygen species (ROS) and EW-7197 suppresses the paclitaxel-induced Snail and EMT by attenuating paclitaxel-induced intracellular ROS. Knock-down of SNAI1 suppresses paclitaxel-induced EMT and CSC properties. These data together suggest that blocking the Snail-induced EMT with the ALK5 inhibitor attenuates metastasis after paclitaxel-therapy and that this combinatorial approach could prove useful in treating breast cancer. | * |
dc.language | English | * |
dc.publisher | IMPACT JOURNALS LLC | * |
dc.subject | paclitaxel | * |
dc.subject | metastasis | * |
dc.subject | Snail | * |
dc.subject | epithelial-to-mesenchymal transition (EMT) | * |
dc.subject | transforming growth factor-beta (TGF-beta) | * |
dc.title | Combinatorial TGF-beta attenuation with paclitaxel inhibits the epithelial-to-mesenchymal transition and breast cancer stem-like cells | * |
dc.type | Article | * |
dc.relation.issue | 35 | * |
dc.relation.volume | 6 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 37535 | * |
dc.relation.lastpage | 37552 | * |
dc.relation.journaltitle | ONCOTARGET | * |
dc.identifier.wosid | WOS:000366113200044 | * |
dc.author.google | Park, So-Yeon | * |
dc.author.google | Kim, Min-Jin | * |
dc.author.google | Park, Sang-A | * |
dc.author.google | Kim, Jung-Shin | * |
dc.author.google | Min, Kyung-Nan | * |
dc.author.google | Kim, Dae-Kee | * |
dc.author.google | Lim, Woosung | * |
dc.author.google | Nam, Jeong-Seok | * |
dc.author.google | Sheen, Yhun Yhong | * |
dc.contributor.scopusid | 신윤용(6603872711) | * |
dc.contributor.scopusid | 김대기(35083694200) | * |
dc.contributor.scopusid | 임우성(27167744500) | * |
dc.date.modifydate | 20240130112620 | * |