Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 심현보 | * |
dc.date.accessioned | 2016-08-27T04:08:35Z | - |
dc.date.available | 2016-08-27T04:08:35Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0006-291X | * |
dc.identifier.issn | 1090-2104 | * |
dc.identifier.other | OAK-16066 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217858 | - |
dc.description.abstract | Tetraspanin 8 (TSPAN8) is a tumor-associated antigen implicated in tumor progression and metastasis. However, the validation of TSPAN8 as a potential therapeutic target in metastatic colorectal cancer (mCRC) has not yet been studied. In this study, through several in vitro methodologies, we identified a large extracellular loop of TSPAN8 (TSPAN8-LEL) as a key domain for regulating mCRC invasion. Using phage display technology, we developed a novel anti-TSPAN8-LEL human antibody with subnanomolar affinity that specifically recognizes amino acids 140-205 of TSPAN8-LEL in a conformation-dependent manner. Finally, we demonstrated that the antibody specifically reduces invasion in the HCT116 and LoVo mCRC cell lines more potently than in the HCT-8 and SW480 non-mCRC cell lines. Our data suggest that TSPAN8-LEL may play an important role in mCRC cell invasion, and that the antibody we have developed could be a useful tool for inhibiting the invasion of TSPAN8-expressing mCRCs. (C) 2015 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | * |
dc.subject | TSPAN8 | * |
dc.subject | Large extracellular loop | * |
dc.subject | Human antibody | * |
dc.subject | Colorectal cancer cells | * |
dc.subject | Invasion | * |
dc.title | Generation of a human antibody that inhibits TSPAN8-mediated invasion of metastatic colorectal cancer cells | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 468 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 774 | * |
dc.relation.lastpage | 780 | * |
dc.relation.journaltitle | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | * |
dc.identifier.doi | 10.1016/j.bbrc.2015.11.031 | * |
dc.identifier.wosid | WOS:000367868500041 | * |
dc.identifier.scopusid | 2-s2.0-84949559092 | * |
dc.author.google | Kim, Taek-Keun | * |
dc.author.google | Park, Chang Sik | * |
dc.author.google | Jeoung, Mee Hyun | * |
dc.author.google | Lee, Woo Ran | * |
dc.author.google | Go, Nam Kyung | * |
dc.author.google | Choi, Jong Rip | * |
dc.author.google | Lee, Tae Sup | * |
dc.author.google | Shim, Hyunbo | * |
dc.author.google | Lee, Sukmook | * |
dc.contributor.scopusid | 심현보(26635827900) | * |
dc.date.modifydate | 20240123110611 | * |