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Estimation of multiexponential fluorescence decay parameters using compressive sensing
- Title
- Estimation of multiexponential fluorescence decay parameters using compressive sensing
- Authors
- Yang, Sejung; Lee, Joohyun; Lee, Youmin; Lee, Minyung; Lee, Byung-Uk
- Ewha Authors
- 이민영; 이병욱; 양세정
- SCOPUS Author ID
- 이민영; 이병욱; 양세정; 양세정
- Issue Date
- 2015
- Journal Title
- JOURNAL OF BIOMEDICAL OPTICS
- ISSN
- 1083-3668
1560-2281
- Citation
- JOURNAL OF BIOMEDICAL OPTICS vol. 20, no. 9
- Keywords
- fluorescence lifetime imaging microscopy; multiexponential decay; fluorescence decay parameters; compressive sensing
- Publisher
- SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Fluorescence lifetime imaging microscopy (FLIM) is a microscopic imaging technique to present an image of fluorophore lifetimes. It circumvents the problems of typical imaging methods such as intensity attenuation from depth since a lifetime is independent of the excitation intensity or fluorophore concentration. The lifetime is estimated from the time sequence of photon counts observed with signal-dependent noise, which has a Poisson distribution. Conventional methods usually estimate single or biexponential decay parameters. However, a lifetime component has a distribution or width, because the lifetime depends on macromolecular conformation or inhomogeneity. We present a novel algorithm based on a sparse representation which can estimate the distribution of lifetime. We verify the enhanced performance through simulations and experiments. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
- DOI
- 10.1117/1.JBO.20.9.096003
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
- Files in This Item:
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