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Effect of Extremely Low Frequency Magnetic Fields on Cell Proliferation and Gene Expression

Title
Effect of Extremely Low Frequency Magnetic Fields on Cell Proliferation and Gene Expression
Authors
Lee, Hyung ChulHong, Mi-NaJung, Seung HeeKim, Bong ChoSuh, Young JuKo, Young-GyuLee, Yun-SilLee, Byeong-YoonCho, Yeun-GyuMyung, Sung-HoLee, Jae-Seon
Ewha Authors
이윤실
SCOPUS Author ID
이윤실scopus
Issue Date
2015
Journal Title
BIOELECTROMAGNETICS
ISSN
0197-8462JCR Link

1521-186XJCR Link
Citation
BIOELECTROMAGNETICS vol. 36, no. 7, pp. 506 - 516
Keywords
ELF-MFcell numbercell viabilityDNA synthesis rateexpression array
Publisher
WILEY-BLACKWELL
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Owing to concerns regarding possible effects of extremely low frequency magnetic fields (ELF-MF) on human health, many studies have been conducted to elucidate whether ELF-MF can induce modifications in biological processes. Despite this, controversies regarding effects of ELF-MF are still rife. In this study, we investigated biological effects of ELF-MF on MCF10A, MCF7, Jurkat, and NIH3T3 cell lines. ELF-MF with a magnetic flux density of 1 mT at 60 Hz was employed to stimulate cells for 4 or 16 h, after which the effects of ELF-MF on cell proliferation, cell death, cell viability, and DNA synthesis rates were assessed. Whereas Jurkat and NIH3T3 cells showed no consistent variation in cell number, cell viability, and DNA synthesis rate, MCF10A and MCF7 cells showed consistent and significant decreases in cell number, cell viability, and DNA synthesis rates. However, there was no effect of ELF-MF on cell death in any of tested cell lines. Next, to investigate the effect of ELF-MF on gene expression, we exposed MCF7 cells to 2 mT at 60 Hz for 16 h and examined transcriptional responses by using gene expression array. We found a gene, PMAIP1, that exhibited statistically significant variation using two-fold cut-off criteria and certified its expression change by using semi-quantitative and quantitative reverse transcription polymerase chain reaction. From these results, we concluded that ELF-MF could induce the delay of cell cycle progression in MCF7 and MCF10A cells in a cell context-specific manner and could up-regulate PMAIP1 in MCF7 cells. (C) 2015 Wiley Periodicals, Inc.
DOI
10.1002/bem.21932
Appears in Collections:
약학대학 > 약학과 > Journal papers
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