MR images of mouse brain using clinical 3T MR scanner and 4CH-Mouse coil

Abstract

Objectives: Although small-bore high-field magnets are useful for research in small rodent models, this technology, however, has not been easily accessible to most researchers. This current study, thus, tried to evaluate the usability of 4CH-Mouse coil (Philips Healthcare, Best, the Netherlands) for preclinical investigations in clinical 3T MR scan environment. We evaluated the effects of ischemic preconditioning (IP) in the mouse stroke model with clinical 3T MR scanner and 4CH-Mouse coil. Materials and Methods: Experiments were performed on male C57BL/6 mice that either received the IP or sham operation (control). Three different MR sequences including diffusion weighted images (DWI), T2-weighted images (T2WI), and fluid attenuated inversion recovery (FLAIR) were performed on the mouse brains following 24, 72 hours of middle cerebral artery occlusion (MCAO) and analyzed for infarct lesions. Results: The images showed that the IP-treated mouse brains had significantly smaller infarct volumes compared to the control group. Of the MR sequences employed, the T2WI showed the highest level of correlations with postmortem infarct volume measurements. Conclusions: The clinical 3T MR scanner turned out to have a solid potential as a practical tool for imaging small animal brains. MR sequences including DWI, T2WI, FLAIR were obtained with acceptable resolution and in a reasonable time constraint in evaluating a mouse stroke model brain.