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dc.contributor.author이공주-
dc.date.accessioned2016-08-27T04:08:52Z-
dc.date.available2016-08-27T04:08:52Z-
dc.date.issued2015-
dc.identifier.issn1949-2553-
dc.identifier.otherOAK-15365-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/217441-
dc.description.abstractThis study explored the role of ubiquitin C-terminal hydrolase-L1 (UCH-L1) in the production of ROS and tumor invasion. UCH-L1 was found to increase cellular ROS levels and promote cell invasion. Silencing UCH-L1, as well as inhibition of H2O2 generation by catalase or by DPI, a NOX inhibitor, suppressed the migration potential of B16F10 cells, indicating that UCH-L1 promotes cell migration by up-regulating H2O2 generation. Silencing NOX4, which generates H2O2, with siRNA eliminated the effect of UCH-L1 on cell migration. On the other hand, NOX4 overexpressed in HeLa cells happens to be ubiquitinated, and NOX4 following deubiquitination by UCH-L1, restored H2O2 -generating activity. These in vitro findings are consistent with the results obtained in vivo with catalase (-/-) C57BL/6J mice. When H2O2 and UCH-L1 levels were independently varied in these animals, the former by infecting with H2O2 -scavenging adenovirus-catalase, and the latter by overexpressing or silencing UCH-L1, pulmonary metastasis of B16F10 cells overexpressing UCH-L1 increased significantly in catalase (-/-) mice. In contrast, invasion did not increase when UCH-L1 was silenced in the B16F10 cells. These findings indicate that H2O2 levels regulated by UCH-L1 are necessary for cell invasion to occur and demonstrate that UCH-L1 promotes cell invasion by up-regulating H2O2 via deubiquitination of NOX4.-
dc.languageEnglish-
dc.publisherIMPACT JOURNALS LLC-
dc.subjectUCH-L1-
dc.subjectubiquitination-
dc.subjecthydrogen peroxide-
dc.subjectNOX4-
dc.subjectinvasion-
dc.titleUbiquitin C-terminal hydrolase-L1 increases cancer cell invasion by modulating hydrogen peroxide generated via NADPH oxidase 4-
dc.typeArticle-
dc.relation.issue18-
dc.relation.volume6-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage16287-
dc.relation.lastpage16303-
dc.relation.journaltitleONCOTARGET-
dc.identifier.wosidWOS:000359012000061-
dc.identifier.scopusid2-s2.0-84937942455-
dc.author.googleKim, Hyun Jung-
dc.author.googleMagesh, Venkataraman-
dc.author.googleLee, Jae-Jin-
dc.author.googleKim, Sun-
dc.author.googleKnaus, Ulla G.-
dc.author.googleLee, Kong-Joo-
dc.contributor.scopusid이공주(7501497635)-
dc.date.modifydate20211116101047-
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약학대학 > 약학과 > Journal papers
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