Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 임재향 | * |
dc.date.accessioned | 2016-08-27T04:08:46Z | - |
dc.date.available | 2016-08-27T04:08:46Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0024-3205 | * |
dc.identifier.issn | 1879-0631 | * |
dc.identifier.other | OAK-15235 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217373 | - |
dc.description.abstract | Aims: bFGF is a potent mitogen of cells associated with fibrosis. Although ERK5 has been reported to play roles in the development of fibrosis, its roles in regulating bFGF-induced fibrotic responses are not understood, especially in lung fibroblasts. The authors investigated the role of ERK5 in bFGF induction of cell proliferation and in induction of PAI-1, a critical regulator of the pathological features of fibrosis, in lung fibroblasts. Main methods: The role played by ERK5 in bFGF-induced PAI-1 expression was elucidated by perturbing the ERK5 signaling pathway using a specific chemical inhibitor and siRNA of ERK5. The effects of ERRS signal perturbation on PAI-1 expression were measured at multiple levels by Q-PCR, immunoblotting, ELISA, and reporter gene analysis. The role of MEF2 in bFGF-induced activation of PAI-1 promoter activity via ERK5 was measured using a biotin-labeled DNA pull-down assay, and the effects of ERK5 on the mitogenic effects of bFGF were assessed using a MIT assay. Key findings: In both primary human lung fibroblast and lung fibroblast cell lines, inhibition of ERK5 blocked bFGF-induced PAI-1 expression at both mRNA and protein levels and inhibited bFGF-induced PAI-1 promoter activity induction by bFGF. Upon stimulation with bFGF, MEF2 directly bound to the consensus sequence of the MEF2 binding site in the PAI-1 promoter. In addition, bFGF-induced PAI-1 up-regulation was inhibited by MEF2 siRNA, and bFGF-induced fibroblast proliferation was blocked by inhibiting ERK5. Significance: This study reveals a novel role for the ERK5-MEF2 cascade, linking bFGF-induced PAI-1 expression and subsequent mitogenic processes in lung fibroblasts. (C) 2015 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | * |
dc.subject | bFGF | * |
dc.subject | ERK5 | * |
dc.subject | PAI-1 | * |
dc.subject | Fibroblasts | * |
dc.subject | Pulmonary fibrosis | * |
dc.title | ERK5 regulates basic fibroblast growth factor-induced type 1 plasminogen activator inhibitor expression and cell proliferation in lung fibroblasts | * |
dc.type | Article | * |
dc.relation.volume | 135 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1 | * |
dc.relation.lastpage | 8 | * |
dc.relation.journaltitle | LIFE SCIENCES | * |
dc.identifier.doi | 10.1016/j.lfs.2015.05.006 | * |
dc.identifier.wosid | WOS:000360028200001 | * |
dc.identifier.scopusid | 2-s2.0-84935000151 | * |
dc.author.google | Han, Jung-Hwa | * |
dc.author.google | Hwang, Ae-Rang | * |
dc.author.google | Nam, Dae-Hwan | * |
dc.author.google | Kim, Suji | * |
dc.author.google | Choi, Hyoung Chul | * |
dc.author.google | Lim, Jae Hyang | * |
dc.author.google | Woo, Chang-Hoon | * |
dc.contributor.scopusid | 임재향(7403454262) | * |
dc.date.modifydate | 20240220101019 | * |