Solubilisation of a 2,2-diphenyl-1-picrylhydrazyl radical in water by beta-cyclodextrin to evaluate the radical-scavenging activity of antioxidants in aqueous media

Abstract

A 2,2-diphenyl-1-picrylhydrazyl radical (DPPH center dot) was successfully solubilised in water by beta-cyclodextrin (beta-CD). DPPH center dot/beta-CD thus obtained was demonstrated to be a powerful tool to evaluate the antioxidative activity of water-soluble antioxidants, such as ascorbate and Trolox, in aqueous buffer solutions.