Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김윤근 | - |
dc.contributor.author | 최준표 | - |
dc.date.accessioned | 2016-08-27T04:08:19Z | - |
dc.date.available | 2016-08-27T04:08:19Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.issn | 1550-6606 | - |
dc.identifier.other | OAK-14717 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217101 | - |
dc.description.abstract | Recent evidence indicates that Gram-negative bacteria-derived extracellular vesicles (EVs) in indoor dust can evoke neutrophilic pulmonary inflammation, which is a key pathology of chronic obstructive pulmonary disease (COPD). Escherichia coli is a ubiquitous bacterium present in indoor dust and secretes nanometer-sized vesicles into the extracellular milieu. In the current study, we evaluated the role of E. coli-derived EVs on the development of COPD, such as emphysema. E. coli EVs were prepared by sequential ultrafiltration and ultracentrifugation. COPD phenotypes and immune responses were evaluated in C57BL/6 wild-type (WT), IFN-gamma-deficient, or IL-17A-deficient mice after airway exposure to E. coli EVs. The present study showed that indoor dust from a bed mattress harbors E. coli EVs. Airway exposure to E. coli EVs increased the production of proinflammatory cytokines, such as TNF-alpha and IL-6. In addition, the repeated inhalation of E. coli EVs for 4 wk induced neutrophilic inflammation and emphysema, which are associated with enhanced elastase activity. Emphysema and elastase activity enhanced by E. coli EVs were reversed by the absence of IFN-gamma or IL-17A genes. In addition, during the early period, lung inflammation is dependent on IL-17A and TNF-alpha, but not on IFN-gamma, and also on TLR4. Moreover, the production of IFN-gamma is eliminated by the absence of IL-17A, whereas IL-17A production is not abolished by IFN-gamma absence. Taken together, the present data suggest that E. coli-derived EVs induce IL-17A-dependent neutrophilic inflammation and thereby emphysema, possibly via upregulation of elastase activity. | - |
dc.language | English | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.title | Extracellular Vesicles Derived from Gram-Negative Bacteria, such as Escherichia coli, Induce Emphysema Mainly via IL-17A-Mediated Neutrophilic Inflammation | - |
dc.type | Article | - |
dc.relation.issue | 7 | - |
dc.relation.volume | 194 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 3361 | - |
dc.relation.lastpage | 3368 | - |
dc.relation.journaltitle | JOURNAL OF IMMUNOLOGY | - |
dc.identifier.doi | 10.4049/jimmunol.1402268 | - |
dc.identifier.wosid | WOS:000351663400041 | - |
dc.author.google | Kim, You-Sun | - |
dc.author.google | Lee, Won-Hee | - |
dc.author.google | Choi, Eun-Jeong | - |
dc.author.google | Choi, Jun-Pyo | - |
dc.author.google | Heo, Young Joo | - |
dc.author.google | Gho, Yong Song | - |
dc.author.google | Jee, Young-Koo | - |
dc.author.google | Oh, Yeon-Mok | - |
dc.author.google | Kim, Yoon-Keun | - |
dc.contributor.scopusid | 김윤근(7410211755;7410198224) | - |
dc.contributor.scopusid | 최준표(34867798100) | - |
dc.date.modifydate | 20230120132215 | - |