One-Photon and Two-Photon Sensing of Biothiols Using a Bis-Pyrene-Cu(II) Ensemble and Its Application To Image GSH in the Cells and Tissues

Abstract

Glutathione (GSH), cysteine (Cys), and homodysteine (Hcy) are three major biothiols, which play key roles in various biological systems. Accordnigly, the development of imaging probes has been actively studied. We report a new pyrene derivative 1, which showed large fluorescence quenching with Cu2+ at pH 7.4. The ensemble 1-Cu2+ was applied to detect iriothiols. Among the various amino acids, GSH, Cys, and Hcy induced distinct turn-on fluorescence changes. The 1-Cu ensemble was further, applied for GSH detection in living cells.