Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김승철 | - |
dc.contributor.author | 이준화 | - |
dc.date.accessioned | 2016-08-27T04:08:50Z | - |
dc.date.available | 2016-08-27T04:08:50Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0172-6390 | - |
dc.identifier.other | OAK-11330 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/216837 | - |
dc.description.abstract | Background/Aims: The aim of this study was to investigate whether profiling metabolic compounds in human colon cancer cells with induced 5-florouracil resistance enables identification of predictive bio-markers for 5-florouracil resistance. Methodology: 5-florouracil resistant and parental cells were extracted using methanol/chloroform solution, and analyzed by MALDI-TOF. Principal components analysis and discriminant analysis was performed to select low-mass ions with strong discriminating power between 5-florouracil resistant and parental cells. The correlation between the intensities of low-mass ions and intrinsic 5-florouracil resistance in 11 colon cancer cells was analyzed using the Spearman rank coefficient. Results: Eleven low-mass ions had strong discriminating power between 5-florouracil-resistant and parental cells. Of these, the intensity of a low-mass ion with 256.29m/z was negatively correlated with intrinsic 5-florouracil resistance in 11 colon cancer cells (r = -0.6545, P = 0.0338). By searching the H+ adduct with 0.05 m/z tolerance in the Human Metabolome Database, a low-mass ion of 256.29 m/z was identified as palmitic amide. Interestingly, extracellular treatment with palmitic amide reduced 5-florouracil resistance and invasiveness in 5-florouracil-resistant cells. Conclusions: Palmitic amide showed potential not only as a predictor of 5-florouracil resistance, but also for reduction of 5-florouracil resistance in colon cancer cells. | - |
dc.language | English | - |
dc.publisher | H G E UPDATE MEDICAL PUBLISHING S A | - |
dc.subject | Palmitic amide | - |
dc.subject | 5-fluorouracil | - |
dc.subject | Colon cancer | - |
dc.subject | Proteomics | - |
dc.title | Decreased Cellular Levels of Palmitic Amide are Linked to 5-fluorouracil Resistance in Human Colon Cancer Cells | - |
dc.type | Article | - |
dc.relation.issue | 130 | - |
dc.relation.volume | 61 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 343 | - |
dc.relation.lastpage | 348 | - |
dc.relation.journaltitle | HEPATO-GASTROENTEROLOGY | - |
dc.identifier.doi | 10.5754/hge13979 | - |
dc.identifier.wosid | WOS:000335085400016 | - |
dc.author.google | Lee, Jun Hwa | - |
dc.author.google | Kim, Seung Cheol | - |
dc.author.google | Kim, Kyung-Hee | - |
dc.author.google | Kim, Won Ki | - |
dc.author.google | Lee, Min Jun | - |
dc.author.google | Yoo, Byong Chul | - |
dc.author.google | Yeo, Seung-Gu | - |
dc.contributor.scopusid | 김승철(35264000100) | - |
dc.contributor.scopusid | 이준화(35181041400) | - |
dc.date.modifydate | 20230210135401 | - |