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The TAM-family receptor Mer mediates production of HGF through the RhoA-dependent pathway in response to apoptotic cells
- Title
- The TAM-family receptor Mer mediates production of HGF through the RhoA-dependent pathway in response to apoptotic cells
- Authors
- Park, Hyun-Jung; Baen, Ji-Yeon; Lee, Ye-Ji; Choi, Youn-Hee; Kang, Jihee Lee
- Ewha Authors
- 이지희; 최윤희
- SCOPUS Author ID
- 이지희; 최윤희
- Issue Date
- 2012
- Journal Title
- MOLECULAR BIOLOGY OF THE CELL
- ISSN
- 1059-1524
- Citation
- MOLECULAR BIOLOGY OF THE CELL vol. 23, no. 16, pp. 3254 - 3265
- Publisher
- AMER SOC CELL BIOLOGY
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- The TAM receptor protein tyrosine kinases Tyro3, Axl, and Mer play important roles in macrophage function. We investigated the roles of the TAM receptors in mediating the induction of hepatocyte growth factor (HGF) during the interaction of macrophages with apoptotic cells. Mer-specific neutralizing antibody, small interfering RNA (siRNA), and a recombinant Mer protein (Mer/Fc) inhibited HGF mRNA and protein expression, as well as activation of RhoA, Akt, and specific mitogen-activated protein (MAP) kinases in response to apoptotic cells. Inhibition of Axl or Tyro3 with specific antibodies, siRNA, or Fc-fusion proteins did not prevent apoptotic cell-induced HGF mRNA and protein expression and did not inhibit activation of the postreceptor signaling molecules RhoA and certain MAP kinases, including extracellular signal-regulated protein kinase and c-Jun NH2-terminal kinase. However, Axl-and Tyro3-specific blockers did inhibit the activation of Akt and p38 MAP kinase in response to apoptotic cells. In addition, none of the TAM receptors mediated the effects of apoptotic cells on transforming growth factor-beta or epidermal growth factor mRNA expression. However, they were involved in the induction of vascular endothelial growth factor mRNA expression. Our data provide evidence that when macrophages interact with apoptotic cells, only Mer of the TAM-family receptors is responsible for mediating transcriptional HGF production through a RhoA-dependent pathway.
- DOI
- 10.1091/mbc.E12-01-0029
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
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