View : 272 Download: 0

Full metadata record

DC Field Value Language
dc.contributor.author강덕희-
dc.contributor.author유민아-
dc.date.accessioned2016-08-27T02:08:06Z-
dc.date.available2016-08-27T02:08:06Z-
dc.date.issued2010-
dc.identifier.issn0250-8095-
dc.identifier.otherOAK-6173-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/216420-
dc.description.abstractBackground: Epithelial-mesenchymal transition (EMT) is important in the development of peritoneal fibrosis. Glucose degradation products (GDPs) may induce EMT in human peritoneal mesothelial cells (HPMCs). Methods: The effects of individual GDPs and GDPs derived from peritoneal dialysis fluid (PDF) in both HPMCs and peritoneal membranes were evaluated. EMT was assessed with alpha-smooth muscle actin (alpha-SMA) and E-cadherin. Results: In vitro, alpha-SMA protein and mRNA levels increased in the presence of the GDPs (formaldehyde, glyoxal, methylglyoxal, and 3-deoxyglucosone), and E-cadherin decreased. Changes in the EMT markers were most prominent after exposure to 3-deoxyglucosone. Changes in both alpha-SMA and E-cadherin protein levels were less with low (L)-GDP bicarbonate/lactate-buffered PDF compared to high (H)-GDP PDF. In the rat model after 8 weeks' PDF infusion, the alpha-SMA/E-cadherin mRNA ratio increased in the H-GDP group compared with the L-GDP group (p < 0.05). The peritoneum in the H-GDP group tended to be thicker (p = 0.052) and had more blood vessels than that in the L-GDP group (p < 0.05). Tissue staining for TGF-beta 1 decreased in the L-GDP group. Dual-stained cytokeratin and alpha-SMA-positive myofibroblasts in the submesothelial layer were more prominent in the H-GDP group. Conclusion: GDPs found in PDF induce EMT of HPMCs, which is associated with peritoneal fibrosis and vascularization. Conversely, L-GDP PDF reduces EMT and peritoneal fibrosis. Copyright (c) 2009 S. Karger AG, Basel-
dc.languageEnglish-
dc.publisherKARGER-
dc.subjectEpithelial-mesenchymal transition-
dc.subjectGlucose degradation products-
dc.subjectVascularization-
dc.subjectPeritoneal fibrosis-
dc.subjectMesothelial cells-
dc.titleImpact of Low Glucose Degradation Product Bicarbonate/Lactate-Buffered Dialysis Solution on the Epithelial-Mesenchymal Transition of Peritoneum-
dc.typeArticle-
dc.relation.issue1-
dc.relation.volume31-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage58-
dc.relation.lastpage67-
dc.relation.journaltitleAMERICAN JOURNAL OF NEPHROLOGY-
dc.identifier.doi10.1159/000256658-
dc.identifier.wosidWOS:000273246300009-
dc.author.googleOh, Eun-Joo-
dc.author.googleRyu, Hye-Myung-
dc.author.googleChoi, Soon-Youn-
dc.author.googleYook, Ju-Min-
dc.author.googleKim, Chan-Duck-
dc.author.googlePark, Sun-Hee-
dc.author.googleChung, Ho-Young-
dc.author.googleKim, In-San-
dc.author.googleYu, Min-A-
dc.author.googleKang, Duk-Hee-
dc.author.googleKim, Yong-Lim-
dc.contributor.scopusid강덕희(17233695600)-
dc.date.modifydate20200911081002-
Appears in Collections:
의과대학 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE