Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박성수 | - |
dc.date.accessioned | 2016-08-27T02:08:27Z | - |
dc.date.available | 2016-08-27T02:08:27Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.other | OAK-3326 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/215989 | - |
dc.description.abstract | We developed a microfluidic immunoassay platform for the decision of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfludic device. Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde. Through this covalent binding, up to 905 mu g immunoglobulin G (IgG) per gram of glass beads was immobilized. For application, glass beads attaching antibody specific to Escherichia coli O157:H7, a foodborne pathogen, were packed into a microfluidic device and used for the detection of the serotype. This prototype immunoassay device can be used for the simulataneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device. | - |
dc.language | English | - |
dc.publisher | KOREAN CHEMICAL SOC | - |
dc.subject | microchamber | - |
dc.subject | glass head | - |
dc.subject | IgG immobilization | - |
dc.subject | pathomilc bacteria | - |
dc.subject | Escherichia coli O157 : H17 | - |
dc.title | Microfluidic immunoassay platform using antibody-immobilized glass beads and its application for detection of Escherichia coli O157 : H7 | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 27 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 479 | - |
dc.relation.lastpage | 483 | - |
dc.relation.journaltitle | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.identifier.wosid | WOS:000237498900005 | - |
dc.identifier.scopusid | 2-s2.0-33646573681 | - |
dc.author.google | Lee, NY | - |
dc.author.google | Yang, Y | - |
dc.author.google | Kim, YS | - |
dc.author.google | Park, S | - |
dc.contributor.scopusid | 박성수(9275920900;41262153700) | - |
dc.date.modifydate | 20230627101106 | - |