View : 667 Download: 0

Redox property and regulation of PpsR, a transcriptional repressor of photosystem gene expression in Rhodobacter sphaeroides

Title
Redox property and regulation of PpsR, a transcriptional repressor of photosystem gene expression in Rhodobacter sphaeroides
Authors
Cho, SHYoun, SHLee, SRYim, HSKang, SO
Ewha Authors
이승록
SCOPUS Author ID
이승록scopus
Issue Date
2004
Journal Title
MICROBIOLOGY-SGM
ISSN
1350-0872JCR Link
Citation
MICROBIOLOGY-SGM vol. 150, pp. 697 - 705
Publisher
SOC GENERAL MICROBIOLOGY
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
PpsR from Rhodobacter sphaeroides is involved in the repression of photosystern gene expression. The PpsR protein was heterologously overexpressed and purified to homogeneity. Gel mobility shift assay showed that the purified PpsR has DNA-binding activity. SDS-PAGE analysis showed that some portions of PpsR were oxidized, indicating that intramolecular or intermolecular disulphide bonds were formed between the two cysteines in each subunit. When the disulphide bond of PpsR was reduced by DTT, the binding activity of PpsR to the puc promoter region distinctly increased. The changes in protein level and DNA-binding activity of PpsR were observed in a conjugant with an extra copy of the ppsR gene and in a PpsR-null mutant (PPS1), respectively. Both cysteines in PpsR existed in their reduced form under aerobic, anaerobic-dark and anaerobic-light growth conditions, as determined using thiol-specific chemical modification. In an AppA-null mutant (APP11), the binding activity and the amount of PpsR decreased compared to those of the wild-type and an appA-complemented strain, and decreased even more under anaerobic-dark conditions than under aerobic conditions. PpsR had a redox-sensitive property but retained its reduced state in the cell, and its amount was reduced by disruption of AppA.
DOI
10.1099/mic.0.26777-0
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

BROWSE