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Genomic organization and expression of parkin in Drosophila melanogaster
- Title
- Genomic organization and expression of parkin in Drosophila melanogaster
- Authors
- Bae, YJ; Park, KS; Kang, SJ
- Ewha Authors
- 강순자
- SCOPUS Author ID
- 강순자
- Issue Date
- 2003
- Journal Title
- EXPERIMENTAL AND MOLECULAR MEDICINE
- ISSN
- 1226-3613
- Citation
- EXPERIMENTAL AND MOLECULAR MEDICINE vol. 35, no. 5, pp. 393 - 402
- Keywords
- ARJP; Drosophila melanogaster; parkin; Parkinson's disease; ubiquitin-ligating enzyme
- Publisher
- KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
- Indexed
- SCIE; SCOPUS; KCI
- Document Type
- Article
- Abstract
- We report here the isolation, characterization on genomic structure and expression of the D. melanogaster homolog of human parkin. The 2,122 bp parkin gene sequence contains six exons that form a 1,449 bp transcript encoding a protein of 482 amino acids. 151 bp of 5' and 112 bp of 3' untranslated regions were identified by a combination of 5'-RACE/primer extension and X-RACE, respectively. The 5' UTR contains three transcription initiation sites. Neither a classical TATA nor a CAAT box was found in the putative promoter sequence. However, binding sites for AhR-Arnt, AP4, NF1 and GATA transcription factors were identified. Transient transfection analysis of the 5' UTR confirmed its promoter activity in HEK 293 cells and SH-SY5Y neuronal cells using a dual luciferase reporting system. The amino acid sequence of D. melanogaster Parkin exhibits 42%, 43% and 43% identity to that of human, mouse and rat, respectively, representing a 54 kDa protein band via western blot analysis. It shows a high degree of conservation in the Ubiquitin-like domain at the N-terminus (34%), the In-Between RING finger domains (IBR, 65-69%), and the RING finger domains at the C-terminus (56-57%). The expression pattern of D. melanogaster parkin varies during the developmental stages, with the highest expression in the adult stage as measured by competitive RT-PCR. From immunostainings of the embryo, D. melanogaster parkin was expressed slightly higher in the central nervous system (brain and nerve cord) during the late embryonic stage.
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- 사범대학 > 과학교육과 > Journal papers
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